通过针对宿主基因的RNA干扰产生的抗烟草花叶病毒烟草。
Tobamovirus-resistant tobacco generated by RNA interference directed against host genes.
作者信息
Asano Momoko, Satoh Rena, Mochizuki Atsuko, Tsuda Shinya, Yamanaka Takuya, Nishiguchi Masamichi, Hirai Katsuyuki, Meshi Tetsuo, Naito Satoshi, Ishikawa Masayuki
机构信息
Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
出版信息
FEBS Lett. 2005 Aug 15;579(20):4479-84. doi: 10.1016/j.febslet.2005.07.021.
Two homologous Nicotiana tabacum genes NtTOM1 and NtTOM3 have been identified. These genes encode polypeptides with amino acid sequence similarity to Arabidopsis thaliana TOM1 and TOM3, which function in parallel to support tobamovirus multiplication. Simultaneous RNA interference against NtTOM1 and NtTOM3 in N. tabacum resulted in nearly complete inhibition of the multiplication of Tomato mosaic virus and other tobamoviruses, but did not affect plant growth or the ability of Cucumber mosaic virus to multiply. As TOM1 and TOM3 homologues are present in a variety of plant species, their inhibition via RNA interference should constitute a useful method for generating tobamovirus-resistant plants.
已鉴定出两个同源的烟草基因NtTOM1和NtTOM3。这些基因编码的多肽与拟南芥TOM1和TOM3的氨基酸序列相似，它们协同发挥作用以支持烟草花叶病毒的繁殖。在烟草中同时对NtTOM1和NtTOM3进行RNA干扰，几乎完全抑制了番茄花叶病毒和其他烟草花叶病毒的繁殖，但不影响植物生长或黄瓜花叶病毒的繁殖能力。由于TOM1和TOM3的同源物存在于多种植物物种中，通过RNA干扰对它们进行抑制应该是一种培育抗烟草花叶病毒植物的有用方法。
Zotero 插件