Han Sang-Hyuk, Park Jong-Seo, Han Jae-Yeong, Gong Jun-Su, Park Chan-Hwan, Kim Jung-Kyu, Seo Eun-Young, Domier Leslie L, Hammond John, Lim Hyoun-Sub
Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 305-764, Korea.
United States Department of Agriculture - Agricultural Research Service, University of Illinois at Urbana-Champaign, Department of Crop Sciences, Champaign, IL, 61801, USA.
Virus Genes. 2017 Jun;53(3):434-445. doi: 10.1007/s11262-017-1432-4. Epub 2017 Feb 7.
Two isolates of Pepper mild mottle virus (PMMoV) were selected from a nationwide survey of pepper fields in South Korea in 2014 and 2015, in which Cucumber mosaic virus was also detected; the two PMMoV isolates, Sangcheong 47 (S-47, KX399390) and Jeongsong 76 (J-76, KX399389), share ~99% nucleotide and amino acid identity and are closely related to Japanese and Chinese isolates at the nucleotide level. Amino acid sequence comparisons revealed 99.73, 99.81, 98.44, and 100% identity in the ORF1, ORF2, MP, and CP, respectively, between S-47 and J-76. In addition, we generated infectious clones of S-47 and J-76, and T7 promoter driven transcripts of each inoculated to Nicotiana benthamiana produced very severe symptoms, whereas only mild symptoms developed in Capsicum annuum. Gene silencing suppressor function of 126 kDa and cytoskeleton-connected plasmodesmata localization of movement protein of S-47 and J-76 showed no difference between isolates, whereas 126 kDa of J-76 clearly formed intracellular aggregates not observed with S-47 126 kDa protein. Differences between these isolates in 126/183 kDa-related functions including subcellular localization suggest that differential interactions with host proteins may affect symptom development in C. annuum.
2014年和2015年在韩国全国范围内对辣椒田进行的调查中，从检测出黄瓜花叶病毒的样本里挑选出了两株辣椒轻斑驳病毒（PMMoV）；这两株PMMoV分离株，桑川47（S-47，KX399390）和井松76（J-76，KX399389），核苷酸和氨基酸同一性约为99%，在核苷酸水平上与日本和中国的分离株密切相关。氨基酸序列比较显示，S-47和J-76之间的ORF1、ORF2、MP和CP的同一性分别为99.73%、99.81%、98.44%和100%。此外，我们构建了S-47和J-76的感染性克隆，并将各自由T7启动子驱动的转录本接种到本氏烟草上，产生了非常严重的症状，而在辣椒上仅出现轻微症状。S-47和J-76的126 kDa基因沉默抑制功能以及运动蛋白与细胞骨架连接的胞间连丝定位在分离株之间没有差异，而J-76的126 kDa明显形成了S-47的126 kDa蛋白未观察到的细胞内聚集体。这些分离株在包括亚细胞定位在内的126/183 kDa相关功能上的差异表明，与宿主蛋白的不同相互作用可能会影响辣椒中的症状发展。
