Lindh Magnus, Hannoun Charles
Department of Clinical Virology, Göteborg University, Guldhedsgatan 10B, 413 46 Göteborg, Sweden.
J Clin Microbiol. 2005 Aug;43(8):4251-4. doi: 10.1128/JCM.43.8.4251-4254.2005.
The Cobas Taqman assay for hepatitis B virus (HBV) DNA showed linear detection over 7 logs for genotypes A to D. The coefficient of variation was 1.2% at > or =1,000 IU/ml and 22.0% at 10 IU/ml. In 97 clinical samples, the log HBV DNA/ml differed by 0.11 between Cobas Amplicor and Cobas Taqman (r2 = 0.97).
用于检测乙型肝炎病毒(HBV)DNA的Cobas Taqman检测法对A至D基因型的检测线性范围超过7个对数级。当浓度≥1000 IU/ml时,变异系数为1.2%;当浓度为10 IU/ml时,变异系数为22.0%。在97份临床样本中,Cobas Amplicor和Cobas Taqman检测法测得的每毫升HBV DNA对数相差0.11(r2 = 0.97)。