Abdel Megeed Kadria N, Abdel Rahman Eman H, Derbala Abdel Meguid A
Department of Parasitology and Animal Diseases National Research Center, Dokki, Giza, Egypt.
J Egypt Soc Parasitol. 2005 Aug;35(2):497-509.
A cross reactive fraction was isolated from hydatid cyst fluid antigen of E. granulosus using CNBr Sepharose 4B affinity chromatography in which anti-T. spiralis antibodies were coupled with the column. Biochemical characterization of the isolated fraction included the use of SDS-PAGE, isoelectric focusing and amino acid analysis. The fraction showed 5 polypeptides of 165, 95.5, 63.5, 30.6 and 24 KDa. The isoelectric points of these polypeptides were 7.8, 7.2, 6.7, 6.2 and 5.7. The fraction exhibited 17 amino acids and was rich in tyrosine (20.81) and glutamic (15.28) ug/100 mg. The fraction proved higher potency in the diagnosis of experimental trichinellosis in rats than echinococcosis in dogs by ELISA. All experimentally infected animals reacted positively, recording 100% diagnostic sensitivity. Collectively, the present study proved that the hydatid cyst fluid cross-reactive fraction could be used in the diagnosis of trichinellosis at different intervals of infection and as early as 1 week post infection.
使用溴化氰琼脂糖4B亲和层析从细粒棘球绦虫包虫囊肿液抗原中分离出一种交叉反应性组分，其中抗旋毛虫抗体与柱结合。对分离出的组分进行生化特性分析，包括使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、等电聚焦和氨基酸分析。该组分显示出165、95.5、63.5、30.6和24 kDa的5种多肽。这些多肽的等电点分别为7.8、7.2、6.7、6.2和5.7。该组分含有17种氨基酸，富含酪氨酸（20.81）和谷氨酸（15.28）μg/100 mg。通过酶联免疫吸附测定（ELISA），该组分在大鼠实验性旋毛虫病诊断中的效力高于犬棘球蚴病诊断中的效力。所有实验感染动物均呈阳性反应，诊断敏感性达100%。总体而言，本研究证明包虫囊肿液交叉反应性组分可用于在感染后的不同时间段以及感染后1周尽早诊断旋毛虫病。
