Abdel-Rahman Eman H, Abdel-Megeed Kadria N, Abuel-Ezz Nadia M T
Department of Parasitiology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
J Egypt Soc Parasitol. 2003 Aug;33(2):457-71.
Cross-reaction between three important zoonotic helminths, Fasciola gigantica, Trichinella spiralis and Echinococcus granulosus, was proved by ELISA. Cross-binding activities in the prepared antisera were strongly directed towards protoscolices and hydatid fluid antigens of E. granulosus rather than to F. gigantica and T. spiralis antigens. Two sets of polypeptides were identified in each antigen by immunoblot; species-specific and cross-reactive. Cross-reactive components in F. gigantica antigen were 205 KD, 178 KD, 166 KD, 106 KD, 100 KD, 65 KD, 45 KD and 32 KD. While, cross-reactive molecules in T. spiralis antigen were 205 KD, 191 KD, 166 KD, 148 KD, 132 KD, and 32 KD. In protoscolices antigen six cross-reactive components were identified, 205 KD, 191 KD, 149 KD, 106 KD, 45 and 32 KD. Moreover, 205 KD, 190 KD, 177 KD, 149 KD, 103 KD and 33 KD were detected in hydatid fluid antigen by heterologous antisera. Interestingly, three polypeptides of 205 KD, 149 KD and 32 KD showed broad immunogenicity with the developed antisera raising the prospect of being putative common immunoprophylactic components.
酶联免疫吸附测定（ELISA）证实了三种重要的人畜共患蠕虫——巨片形吸虫、旋毛虫和细粒棘球绦虫之间存在交叉反应。所制备抗血清中的交叉结合活性主要针对细粒棘球绦虫的原头节和包虫囊液抗原，而非巨片形吸虫和旋毛虫抗原。通过免疫印迹法在每种抗原中鉴定出两组多肽：种特异性多肽和交叉反应性多肽。巨片形吸虫抗原中的交叉反应成分有205千道尔顿（KD）、178 KD、166 KD、106 KD、100 KD、65 KD、45 KD和32 KD。而旋毛虫抗原中的交叉反应分子有205 KD、191 KD、166 KD、148 KD、132 KD和32 KD。在原头节抗原中鉴定出六种交叉反应成分，即205 KD、191 KD、149 KD、106 KD、45 KD和32 KD。此外，用异种抗血清在包虫囊液抗原中检测到205 KD、190 KD、177 KD、149 KD、103 KD和33 KD。有趣的是，205 KD、149 KD和32 KD这三种多肽对所制备的抗血清显示出广泛的免疫原性，这增加了它们成为假定的共同免疫预防成分的可能性。
