Abdel Megeed Kadria N, Abuel Ezz Nadia M T, Abdel-Rahman Eman H
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
J Egypt Soc Parasitol. 2005 Aug;35(2):581-95.
Immunization of rabbits against hepatic coccidiosis was tried. The animals were immunized twice with Eimeria stiedae coproantigen in freund's adjuvant with two week intervals. The rabbits were challenged orally with sporulated E. stiedae oocysts one week post last injection. The protection was assessed by number of oocysts output, number of focal liver lesions, clinical sings and antibody response. The immunization resulted in 70% protection from infection and decline in oocysts count. High level of IgG response in immunized rabbits than control infected ones was occurred and being responsible for the recorded protection. The electrophoretic make up of the coproantigen and oocyst antigen showed different patterns of separation. Common as well as specific bands to each antigen were identified. Using the rabbit sera after 3 weeks post vaccination in immunoblot assay, immunogenic components were detected of molecular weight 155, 103, 74, 66, 44, 22KD with coproantigen and 155, 115, 57, 26 KD with oocyst antigen. While, the rabbit sera after 2 and 4 weeks post challenge reacted with oocyts antigen, in immunoblot assay, revealing immunogenic bands of molecular weight 155, 115, 57, 26, 22KD and 155, 115, 57, 25 KD respectively. Bands of 22KD and 155KD are partially responsible for eliciting host protective immune response where they were recognized by immunized sera in coproantigen and by immunized infected sera in oocyst antigen.
尝试对家兔进行抗肝球虫病免疫。用斯氏艾美耳球虫粪便抗原与弗氏佐剂对动物进行两次免疫，间隔两周。在最后一次注射后一周，给家兔口服感染有孢子化的斯氏艾美耳球虫卵囊。通过卵囊排出数量、肝脏病灶数量、临床症状和抗体反应来评估保护效果。免疫接种产生了70%的感染保护率，并使卵囊数量减少。免疫家兔的IgG反应水平高于对照感染家兔，这是所记录的保护效果的原因。粪便抗原和卵囊抗原的电泳组成显示出不同的分离模式。鉴定出了每种抗原的共同条带以及特异性条带。在免疫印迹分析中，使用接种疫苗3周后的家兔血清，检测到与粪便抗原反应的分子量为155、103、74、66、44、22KD的免疫原性成分，以及与卵囊抗原反应的分子量为155、115、57、26KD的免疫原性成分。而在攻击后2周和4周的家兔血清在免疫印迹分析中与卵囊抗原反应，分别显示出分子量为155、115、57、26、22KD和155、115、57、25KD的免疫原性条带。分子量为22KD和155KD的条带部分负责引发宿主保护性免疫反应，在粪便抗原中它们被免疫血清识别，在卵囊抗原中被免疫感染血清识别。
