Song Hongyan, Dong Ronglian, Qiu Baofeng, Jing Jin, Zhu Shunxing, Liu Chun, Jiang Yingmei, Wu Liucheng, Wang Shengcun, Miao Jin, Shao Yixiang
Laboratory Animal Center of Nantong University, Nantong 226001, China.
Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 21009, China.
Korean J Parasitol. 2017 Feb;55(1):15-20. doi: 10.3347/kjp.2017.55.1.15. Epub 2017 Feb 28.
The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of . Protein spots were recognized by the sera of New Zealand rabbits infected artificially with . The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti- sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with , which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.
本研究的目的是鉴定用于控制兔球虫病的疫苗或药物靶点的抗原。采用二维电泳、免疫印迹和质谱分析相结合的方法，从[未提及的寄生虫名称]子孢子中鉴定新抗原。蛋白质斑点通过人工感染[未提及的寄生虫名称]的新西兰兔血清进行识别。结合生物信息学，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/TOF-MS）分析对蛋白质进行表征。通过银染检测到约868个蛋白质斑点，抗血清识别出总共41个免疫反应性蛋白质斑点。最终成功鉴定出23个蛋白质斑点。热休克蛋白70和天冬氨酰蛋白酶等蛋白质可能具有作为免疫诊断或疫苗抗原的潜力。发现这些免疫反应性蛋白质具有广泛的生物学功能。本研究首次报道了感染[未提及的寄生虫名称]的兔血清所识别的蛋白质，这可能有助于确定控制兔球虫病疫苗开发的潜在靶点。
