Whelan J, Broughton K S, Surette M E, Kinsella J E
Institute of Food Science, Cornell University, Ithaca, New York 14853.
Lipids. 1992 Jan;27(1):85-8. doi: 10.1007/BF02537068.
The effects of preformed dietary arachidonic acid (AA, 20:4n-6) on murine phospholipid fatty acid composition in tissues capable (liver) and incapable (peritoneal exudate cells, PEC) of desaturating and elongating linoleic acid (LA, 18:2n-6) to AA were investigated. The results were compared with those obtained on matched animals on LA diets by either substituting or supplementing dietary LA with AA. Modest amounts of AA ethyl ester (0.5 wt%) included in the diet significantly increased tissue phospholipid AA levels by 39% and 57% in the liver and in PEC, respectively. The changes were further enhanced when dietary LA and AA intakes were equivalent, i.e., 57% and 68% in liver and PEC, respectively. This enrichment was observed in all phospholipid classes analyzed, with the greatest impact on phosphatidylcholine. In addition, the doubling of dietary LA had little effect on tissue phospholipid AA levels. The data suggest that while the level of n-6 PUFA may have an important effect on tissue fatty acid composition, the type of n-6 PUFA in the diet could be of greater significance.
研究了预先形成的膳食花生四烯酸（AA，20:4n-6）对能够（肝脏）和不能够（腹腔渗出细胞，PEC）将亚油酸（LA，18:2n-6）去饱和并延长为AA的组织中鼠磷脂脂肪酸组成的影响。将结果与通过用AA替代或补充膳食LA在匹配的以LA为食的动物上获得的结果进行比较。饮食中包含适量的AA乙酯（0.5 wt%）分别使肝脏和PEC中的组织磷脂AA水平显著提高了39%和57%。当膳食LA和AA摄入量相等时，即肝脏和PEC中分别为57%和68%时，这种变化进一步增强。在所有分析的磷脂类别中都观察到了这种富集，对磷脂酰胆碱的影响最大。此外，膳食LA加倍对组织磷脂AA水平几乎没有影响。数据表明，虽然n-6多不饱和脂肪酸的水平可能对组织脂肪酸组成有重要影响，但饮食中n-6多不饱和脂肪酸的类型可能更具意义。
