Reyzer Michelle L, Caprioli Richard M
Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
J Proteome Res. 2005 Jul-Aug;4(4):1138-42. doi: 10.1021/pr050095+.
The direct analysis of tissue sections by MALDI mass spectrometry holds tremendous potential for biomarker discovery. This technology routinely allows many hundreds of proteins to be detected over a mass range of approximately 2000-70 000 Da while maintaining the spatial localization of the proteins detected. This technology has been applied to a wide range of tissue samples, including human glioma tissue and human lung tumor tissue. In many cases, biostatistical analyses of the resulting protein profiles revealed patterns that correlated with disease state and/or clinical endpoints. This work serves as a review of recent applications and summarizes the current state of technology.
通过基质辅助激光解吸电离质谱法（MALDI）对组织切片进行直接分析在生物标志物发现方面具有巨大潜力。这项技术通常能够在大约2000 - 70000道尔顿的质量范围内检测到数百种蛋白质，同时保持所检测蛋白质的空间定位。该技术已应用于广泛的组织样本，包括人类胶质瘤组织和人类肺癌组织。在许多情况下，对所得蛋白质谱进行的生物统计学分析揭示了与疾病状态和/或临床终点相关的模式。本文对近期的应用进行了综述，并总结了当前的技术状态。
