Tyan Yu-Chang, Wu Hsin-Yi, Lai Wu-Wei, Su Wu-Chou, Liao Pao-Chi
Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, 138 Sheng-Li Road, Tainan 704, Taiwan.
J Proteome Res. 2005 Jul-Aug;4(4):1274-86. doi: 10.1021/pr049746c.
Pleural effusion, an accumulation of pleural fluid, contains proteins originated from plasma filtrate and, especially when tissues are damaged, parenchyma interstitial spaces of lungs and/or other organs. This study details protein profiles in human pleural effusion from 43 lung adenocarcinoma patients by a two-dimensional nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (2D nano-HPLC-ESI-MS/MS) system. The experimental results revealed the identification of 1415 unique proteins from human pleural effusion. Among these 124 proteins identified with higher confidence levels, some proteins have not been reported in plasma and may represent proteins specifically present in pleural effusion. These proteins are valuable for mass identification of differentially expressed proteins involved in proteomics database and screening biomarker to further study in human lung adenocarcinoma. The significance of the use of proteomics analysis of human pleural fluid for the search of new lung cancer marker proteins, and for their simultaneous display and analysis in patients suffering from lung disorders has been examined.
胸腔积液是胸膜液的积聚，其中含有源自血浆滤液的蛋白质，特别是当组织受损时，还含有肺和/或其他器官的实质间质间隙中的蛋白质。本研究通过二维纳米高效液相色谱电喷雾电离串联质谱（2D纳米HPLC-ESI-MS/MS）系统详细分析了43例肺腺癌患者胸腔积液中的蛋白质谱。实验结果显示，从人胸腔积液中鉴定出1415种独特蛋白质。在这124种鉴定可信度较高的蛋白质中，有些蛋白质在血浆中尚未见报道，可能代表胸腔积液中特有的蛋白质。这些蛋白质对于蛋白质组学数据库中差异表达蛋白质的大规模鉴定以及筛选生物标志物以进一步研究人类肺腺癌具有重要价值。研究了利用人类胸水蛋白质组学分析寻找新的肺癌标志物蛋白，以及在肺部疾病患者中同时展示和分析这些蛋白的意义。
