Mignogna Giuseppina, Giorgi Alessandra, Stefanelli Paola, Neri Arianna, Colotti Gianni, Maras Bruno, Schininà M Eugenia
Dipartimento di Scienze Biochimiche, Università La Sapienza, Piazzale A. Moro 5, I-00185 Rome, Italy.
J Proteome Res. 2005 Jul-Aug;4(4):1361-70. doi: 10.1021/pr0500511.
A protein inventory of Neisseria meningitidis strain MC58, a meningococcal strain belonging to the serogroup B, was performed by proteomics. A differential extraction procedure was employed and 238 protein species were identified by 2D mini-maps and MALDI-ToF analyses. In this catalog, we detected protein products from 33 genes, which were not yet annotated in previous N. meningitidis proteomic studies. This approach is suitable for high-throughput studies on differential expression of N. meningitidis genomes.
采用蛋白质组学方法对属于B血清群的脑膜炎奈瑟菌菌株MC58进行了蛋白质鉴定。采用了差异提取程序，并通过二维微图谱和基质辅助激光解吸电离飞行时间（MALDI-ToF）分析鉴定出238种蛋白质。在该目录中，我们检测到了来自33个基因的蛋白质产物，这些基因在之前的脑膜炎奈瑟菌蛋白质组学研究中尚未注释。这种方法适用于脑膜炎奈瑟菌基因组差异表达的高通量研究。
