García-Tomás M, Sánchez J, Rafel O, Ramon J, Piles M
IRTA-Unitat de Cunicultura, Torre Marimón s/n., 08140 Caldes de Montbuí, Barcelona, Spain.
Anim Reprod Sci. 2006 Jun;93(1-2):88-100. doi: 10.1016/j.anireprosci.2005.06.011. Epub 2005 Aug 3.
A total of 2140 ejaculates from 156 adult males pertaining to four groups of bucks were analysed and the following traits were recorded: pH, ejaculate volume (V), mass motility (Mm), individual motility (Mi), concentration (Cn), total number of spermatozoa per ejaculate (TSE), percentage of sperm viability (Vi), percentage of sperm with acrosome integrity (NAR), percentage of sperm normalcy (Nr), percentage of sperm morphological abnormalities of head (H), neck-midpiece (Nm) and tail (T) and presence of proximal and distal cytoplasmic droplet (Dp, Dd). Principal component (PC) analysis and phenotypic correlations were performed in order to examine the relationships between qualitative and quantitative traits of rabbit semen. The repeatability of the traits measured was also estimated. Phenotypic correlations between sperm traits were estimated as the residual correlation from an analysis of variance, including the effects of: genetic type of the male, order of the ejaculate, day of collection and the permanent non-additive random effect of the male to which the observation corresponds. The repeatability of these traits was analysed separately, in a set of univariate analyses, using VCE software. The previously defined mixed model was used for this analysis. The principal component analysis was performed using the Princomp procedure of the SAS v.8 package. The first four PC explained 62% of total variation: 23%, 18%, 12% and 8%, respectively. Percentage of sperm viability, NAR, Nr, T and Nm were the predominant variables in the first PC. Mass motility, Mi, pH, Cn and TSE were located in the second. Percentage of sperm with the presence of proximal and distal cytoplasmic droplet, NAR, Vi and Nr were the predominant traits in the third and V defined the fourth. Repeatability of semen quality traits was moderate for most of the traits. The highest values (about 0.45) corresponded to V, Cn, Nr and Dp and the lowest (about 0.10) to H and Nm. Repeatability of the first ejaculate was greater than that of the second for pH, Mm, Mi, H, Dp and Dd.
对来自四组成年雄鹿的156只成年雄性的总共2140份射精样本进行了分析，并记录了以下特征：pH值、射精量（V）、总体活力（Mm）、个体活力（Mi）、浓度（Cn）、每次射精的精子总数（TSE）、精子活力百分比（Vi）、顶体完整精子百分比（NAR）、精子正常率（Nr）、头部（H）、颈部 - 中段（Nm）和尾部（T）精子形态异常百分比以及近端和远端细胞质滴的存在情况（Dp，Dd）。进行主成分（PC）分析和表型相关性分析，以研究兔精液定性和定量特征之间的关系。还估计了所测性状的重复性。精子性状之间的表型相关性通过方差分析的残差相关性来估计，方差分析包括以下因素的影响：雄性的遗传类型、射精顺序、采集日期以及与观察对应的雄性的永久非加性随机效应。使用VCE软件在一组单变量分析中分别分析这些性状的重复性。此分析使用先前定义的混合模型。主成分分析使用SAS v.8软件包的Princomp程序进行。前四个主成分解释了总变异的62%：分别为23%、18%、12%和8%。精子活力百分比、NAR、Nr、T和Nm是第一主成分中的主要变量。总体活力、Mi、pH值、Cn和TSE位于第二主成分中。有近端和远端细胞质滴的精子百分比、NAR、Vi和Nr是第三主成分中的主要性状，而V定义了第四主成分。大多数精液质量性状的重复性适中。最高值（约0.45）对应于V、Cn、Nr和Dp，最低值（约0.10）对应于H和Nm。pH值、Mm、Mi、H、Dp和Dd的第一次射精的重复性大于第二次射精。
