Morris Lee H A
EquiBreed Ltd, Matamata, New Zealand.
Anim Reprod Sci. 2005 Oct;89(1-4):147-57. doi: 10.1016/j.anireprosci.2005.06.024.
Since the production of the first live offspring from sex-sorted spermatozoa in 1989, there have been many developments in the fluorescence-activated cell separation (FACS) procedures to preselect X- and Y-chromosome bearing spermatozoa prior to insemination. During this time, FACS technology has been applied to a range of species and has resulted in offspring from rabbits, cattle, sheep, elk and horses. In horses, satisfactory fertility rates have been achieved after hysteroscopic insemination of 20 x 10(6) fresh or stored, sex-sorted spermatozoa. However, many of the sperm processing protocols are still based on the original protocol and components of these procedures may not necessarily be suitable for the stallion. This review examines the details of FACS protocols that have resulted in the production of live offspring and makes comparisons with the published stallion protocols in an attempt to determine how best to improve the fertility of sorted, frozen-thawed stallion spermatozoa.
自1989年首次利用性别分选精子产生活体后代以来，在受精前预选携带X和Y染色体精子的荧光激活细胞分选（FACS）程序方面有了许多进展。在此期间，FACS技术已应用于一系列物种，并产生了兔、牛、羊、麋鹿和马的后代。在马中，经宫腔镜授精20×10⁶新鲜或冷冻保存的性别分选精子后，已获得令人满意的受胎率。然而，许多精子处理方案仍基于原始方案，这些程序的组成部分不一定适用于种马。本综述研究了已产生活体后代的FACS方案的细节，并与已发表的种马方案进行比较，试图确定如何最好地提高分选的、冻融种马精子的受胎率。
