Petrella Carolina, Hsieh Jennifer, Thrift Kim, Jarow Jonathan P, Zacur Howard, Zhao Yulian
Department of Obstetrics and Gynecology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Fertil Steril. 2005 Aug;84(2):513-5. doi: 10.1016/j.fertnstert.2005.01.138.
To determine the optimal conditions to maintain sperm motility and viability for prolonged periods, semen specimens were processed and cultured in different temperature and conditions. We conclude that processing of semen and incubation of sperm in protein-supplemented N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) human tubal fluid medium at room temperature best preserved viability and motility of sperm.