Brinsko S P, Van Wagner G S, Graham J K, Squires E L
Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523, USA.
J Reprod Fertil Suppl. 2000(56):111-20.
The aim of the present study was to determine whether there are characteristics of fresh, cooled and frozen-thawed semen samples that can be used to predict the suitability of stallion semen for preservation by cooling or freezing. Each of three ejaculates obtained from 12 stallions was divided into aliquots to be analysed for sperm motility, morphology and membrane integrity as fresh, cooled and frozen-thawed samples. The percentage of morphologically normal spermatozoa was similar in fresh and cooled samples and both were greater than in the frozen samples. There were no strong linear relationships in the percentages of progressive motility, live spermatozoa and morphologically normal spermatozoa in fresh, cooled and frozen-thawed semen samples. Although the percentages of motile spermatozoa in fresh, cooled and frozen samples were linearly related, when sperm motility was ranked as excellent, good, fair and poor, the only correlation observed was between progressive sperm motility in fresh samples and total sperm motility in frozen samples. The results of the present study demonstrate that the high percentage of progressive motility in fresh semen samples is not indicative of similar patterns in cooled or frozen-thawed samples. Commonly used methods for assessing sperm function do not appear to be useful for predicting the ability of stallion semen to withstand preservation by either cooling or freezing.
本研究的目的是确定新鲜、冷却和冻融精液样本是否具有可用于预测种马精液通过冷却或冷冻保存的适用性的特征。从12匹种马获得的三份射精样本中的每一份都被分成若干小份，作为新鲜、冷却和冻融样本进行精子活力、形态和膜完整性分析。新鲜和冷却样本中形态正常的精子百分比相似，且两者均高于冷冻样本。新鲜、冷却和冻融精液样本中进行性运动精子、活精子和形态正常精子的百分比之间没有很强的线性关系。尽管新鲜、冷却和冷冻样本中活动精子的百分比呈线性相关，但当将精子活力分为优秀、良好、中等和差时，观察到的唯一相关性是新鲜样本中的进行性精子活力与冷冻样本中的总精子活力之间的相关性。本研究结果表明，新鲜精液样本中高比例的进行性运动能力并不表明冷却或冻融样本中具有相似模式。常用的评估精子功能的方法似乎无助于预测种马精液耐受冷却或冷冻保存的能力。
