Redei E
Department of Psychiatry, University of Pennsylvania, Philadelphia 19104-6141.
Neuroendocrinology. 1992 Jan;55(1):115-8. doi: 10.1159/000126104.
Immunoreactive corticotropin-releasing factor (CRF) was identified and measured by radioimmunossay in rat thymic extract. Serial dilutions of thymic extract paralleled hypothalamic extract and synthetic CRF standard curves. CRF extracted from rat thymus eluted in the position of synthetic rat CRF on Sephadex G-25 column. Thymus-derived CRF was biologically active as demonstrated by stimulating adrenocorticotropic hormone secretion in dispersed rat anterior pituitary cells. These findings indicate the presence of authentic CRF in rat thymus, further supporting the concept of an integrated regulation of the neuroendocrine-immune responses to environmental stimuli.
采用放射免疫分析法在大鼠胸腺提取物中鉴定并测定了免疫反应性促肾上腺皮质激素释放因子（CRF）。胸腺提取物的系列稀释液与下丘脑提取物及合成CRF标准曲线平行。从大鼠胸腺中提取的CRF在Sephadex G - 25柱上的洗脱位置与合成大鼠CRF相同。胸腺来源的CRF具有生物活性，这在分散的大鼠垂体前叶细胞中刺激促肾上腺皮质激素分泌得到了证实。这些发现表明大鼠胸腺中存在真正的CRF，进一步支持了对环境刺激的神经内分泌 - 免疫反应进行综合调节的概念。
