Relova Anne-Jacqueline, Shahana Shahida, Makeeva Natalia, Roomans Godfried M
Department of Medical Cell Biology, University of Uppsala, Box 571, SE-75 123 Uppsala, Sweden.
Cell Biol Int. 2005 Sep;29(9):768-77. doi: 10.1016/j.cellbi.2005.05.002.
The presence of adhesion molecules on airway epithelial cells may be important in recruiting leukocytes to the epithelium. The study aimed at investigating the effects of interleukin (IL)-4, IL-8, IL-13 and interferon-gamma (IFN-gamma) on cell viability and intracellular adhesion molecule (ICAM)-1 and zonula occludens protein (ZO)-1 expression on cultured human basal and columnar airway epithelial cells. Cycloheximide (CHX) induced cell death in both cell lines. The cytokines IL-4, IL-8, IL-13 and IFN-gamma had only minor effects on cell proliferation in the columnar 16HBE14o-cells, and inhibited the effects of CHX on cell death. IFN-gamma increased ICAM-1 expression in both cell lines. Western blot analysis showed that CHX inhibited both ICAM-1 and ZO-1 expression in the basal cell line. A combination of IL-4 and IFN-gamma appeared to break the tight junctions. IL-4 and IL-13 potentiated CHX-induced apoptosis in basal cells but not in columnar cells, possibly due to low levels of the IL-4 receptor. It is concluded that cytokines produced by airway epithelium may have a role in regulating sequestering of leukocytes to the airways during airway inflammation.
气道上皮细胞上黏附分子的存在可能在将白细胞募集到上皮细胞过程中起重要作用。该研究旨在调查白细胞介素（IL）-4、IL-8、IL-13和干扰素-γ（IFN-γ）对培养的人基底和柱状气道上皮细胞的细胞活力以及细胞间黏附分子（ICAM）-1和紧密连接蛋白（ZO）-1表达的影响。放线菌酮（CHX）可诱导两种细胞系发生细胞死亡。细胞因子IL-4、IL-8、IL-13和IFN-γ对柱状16HBE14o-细胞的细胞增殖仅有轻微影响，并抑制CHX对细胞死亡的作用。IFN-γ可增加两种细胞系中ICAM-1的表达。蛋白质免疫印迹分析显示，CHX可抑制基底细胞系中ICAM-1和ZO-1的表达。IL-4和IFN-γ的组合似乎会破坏紧密连接。IL-4和IL-13可增强CHX诱导的基底细胞凋亡，但对柱状细胞无此作用，这可能是由于IL-4受体水平较低所致。研究得出结论，气道上皮产生的细胞因子可能在气道炎症期间调节白细胞向气道的聚集过程中发挥作用。
