Qiao Shanlou, Li Weihua, Tsubouchi Ryoko, Haneda Miyako, Murakami Keiko, Takeuchi Fumio, Nisimoto Yukio, Yoshino Masataka
Department of Biochemistry, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195, Japan.
Free Radic Res. 2005 Sep;39(9):995-1003. doi: 10.1080/10715760500231836.
Antioxidant action of Rosmarinic acid (Ros A), a natural phenolic ingredient in many Lamiaceae herbs such as Perilla frutescens, sage, basil and mint, was analyzed in relation to the Ikappa-B activation in RAW264.7 macrophages. Ros A inhibited nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) protein synthesis induced by lipopolysaccharide (LPS), and also effectively suppressed phorbol 12-myristate 13-acetate (PMA)-induced superoxide production in RAW264.7 macrophages in a dose-dependent manner. Peroxynitrite-induced formation of 3-nitrotyrosine in bovine serum albumin and RAW264.7 macrophages were also inhibited by Ros A. Moreover, Western blot analysis demonstrated that LPS-induced phosphorylation of Ikappa-Balpha was abolished by Ros A. Ros A can act as an effective protector against peroxynitrite-mediated damage, and as a potent inhibitor of superoxide and NO synthesis; the inhibition of the formation of reactive oxygen and nitrogen species are partly based on its ability to inhibit the serine phosphorylation of Ikappa-Balpha.
迷迭香酸（Ros A）是紫苏、鼠尾草、罗勒和薄荷等许多唇形科草本植物中的一种天然酚类成分，研究了其抗氧化作用与RAW264.7巨噬细胞中Ikappa-B激活的关系。Ros A抑制脂多糖（LPS）诱导的一氧化氮（NO）产生和诱导型一氧化氮合酶（iNOS）蛋白合成，并且还以剂量依赖的方式有效抑制佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）诱导的RAW264.7巨噬细胞中的超氧化物产生。Ros A还抑制过氧亚硝酸盐诱导的牛血清白蛋白和RAW264.7巨噬细胞中3-硝基酪氨酸的形成。此外，蛋白质印迹分析表明，Ros A消除了LPS诱导的Ikappa-Balpha磷酸化。Ros A可以作为过氧亚硝酸盐介导的损伤的有效保护剂，以及超氧化物和NO合成的有效抑制剂；对活性氧和氮物种形成的抑制部分基于其抑制Ikappa-Balpha丝氨酸磷酸化的能力。
