Han Qing-min, Xie Jie, Chai Sheng-ting, Fang Jian, Liu Qingsi
The Third Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Guangzhou.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2005 Jul;25(7):637-9.
To investigate effects of electro-acupuncture (EA) on Du Meridian on the water channel aquaporin-4 (AQP-4) expression and hind limbs function recovery in experimental spinal cord injured rats.
One hundred and fifty SD rats were divided into the normal group, the model group and the EA group, with 50 rats in each group, modified Allen method was used to establish spinal cord injury model in the model and EA group. Rats in the EA group was EA on Dazhui and Ming men acupoints after modelling successfully. At the 1st, 3rd, 7th, 14th and 21th day after injury, the hind limbs function of rats was evaluated by BBB scales respectively, and the AQP-4 expression in spinal cord tissue was determined by immunohistochemistry technique and quantity analysed with image analyzer.
At the 1st day after spinal cord injury, the AQP-4 expression was significantly increased in gray matter and white matter of spinal cord in the model group and the EA group, which reached the peak at the 3rd day, but showed significant difference between the two groups (P < 0.05), and the difference at the 7th, 14th and 21th day became more significant (P < 0.01).
EA on Du Meridian can down-regulate AQP-4 expression after spinal cord injury, inhibit spinal cord edema for alleviating secondary spinal cord lesion, so as to protect the residual normal spinal cord tissues and promote the rebuilding of nervous tissues.
探讨督脉电针（EA）对实验性脊髓损伤大鼠水通道蛋白4（AQP-4）表达及后肢功能恢复的影响。
将150只SD大鼠分为正常组、模型组和电针组，每组50只。模型组和电针组采用改良Allen法建立脊髓损伤模型。电针组大鼠造模成功后于大椎穴和命门穴行电针治疗。于伤后第1、3、7、14和21天，分别采用BBB评分评估大鼠后肢功能，采用免疫组织化学技术检测脊髓组织中AQP-4表达，并利用图像分析仪进行定量分析。
脊髓损伤后第1天，模型组和电针组脊髓灰质和白质中AQP-4表达均显著增加，于第3天达到峰值，但两组间差异显著（P<0.05），第7、14和21天差异更显著（P<0.01）。
督脉电针可下调脊髓损伤后AQP-4表达，抑制脊髓水肿以减轻继发性脊髓损伤，从而保护残余正常脊髓组织并促进神经组织重建。
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