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一种共激活因子的鉴定，该因子在体外可增强血清反应因子和GAL4-VP16介导的转录激活作用。

Identification of a coactivator that increases activation of transcription by serum response factor and GAL4-VP16 in vitro.

作者信息

Zhu H, Pyrwes R

机构信息

Department of Biological Sciences, Columbia University, New York, NY 10027.

出版信息

Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5291-5. doi: 10.1073/pnas.89.12.5291.

DOI:10.1073/pnas.89.12.5291
PMID:1608937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC49277/
Abstract

We have identified an activity in HeLa cell nuclear extracts that increases activation of transcription in vitro by serum response factor (SRF). This coactivator activity, termed CoS, did not affect basal (TATA only) transcription. CoS initially fractionated with transcription factors TFIIE and -F but was further purified to separate it from both TFIIE and -F as well as any other general transcription factor activity. We found that CoS was not specific for SRF activation, since it also increased transcriptional activation by the chimeric GAL4-VP16 activator. Differences among CoS and recently identified coactivators are discussed.

摘要

我们在HeLa细胞核提取物中鉴定出一种活性物质，它能增强血清反应因子（SRF）在体外的转录激活作用。这种共激活因子活性，称为CoS，并不影响基础（仅TATA）转录。CoS最初与转录因子TFIIE和-F一起分离，但经过进一步纯化，使其与TFIIE和-F以及任何其他一般转录因子活性分开。我们发现CoS对SRF激活不具有特异性，因为它也能增强嵌合型GAL4-VP16激活因子的转录激活作用。文中讨论了CoS与最近鉴定出的共激活因子之间的差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/bf4b353d37c8/pnas01086-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/2bfa213e1e75/pnas01086-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/5fcc8ec79edc/pnas01086-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/2f5cc014ab85/pnas01086-0101-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/bf4b353d37c8/pnas01086-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/2bfa213e1e75/pnas01086-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/5fcc8ec79edc/pnas01086-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/2f5cc014ab85/pnas01086-0101-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b67/49277/bf4b353d37c8/pnas01086-0102-a.jpg

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本文引用的文献

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