Brodie Of Brodie E B, Nicolay S, Touchon M, Audit B, d'Aubenton-Carafa Y, Thermes C, Arneodo A
Laboratoire Joliot-Curie (CNRS), Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon Cedex 07, France.
Phys Rev Lett. 2005 Jun 24;94(24):248103. doi: 10.1103/PhysRevLett.94.248103. Epub 2005 Jun 23.
We explore the large-scale behavior of nucleotide compositional strand asymmetries along human chromosomes. As we observe for 7 of 9 origins of replication experimentally identified so far, the (TA+GC) skew displays rather sharp upward jumps, with a linear decreasing profile in between two successive jumps. We present a model of replication with well positioned replication origins and random terminations that accounts for the observed characteristic serrated skew profiles. We succeed in identifying 287 pairs of putative adjacent replication origins with an origin spacing approximately 1-2 Mbp that are likely to correspond to replication foci observed in interphase nuclei and recognized as stable structures that persist throughout subsequent cell generations.
我们探究了沿人类染色体核苷酸组成链不对称性的大规模行为。正如我们目前通过实验鉴定出的9个复制起点中的7个所观察到的那样，（TA+GC）偏斜呈现出相当明显的向上跳跃，在两个连续跳跃之间呈线性下降趋势。我们提出了一个具有定位良好的复制起点和随机终止的复制模型，该模型解释了观察到的特征性锯齿状偏斜图谱。我们成功识别出287对假定的相邻复制起点，其起点间距约为1-2兆碱基对，这些起点可能对应于在间期细胞核中观察到的复制焦点，并被认为是在随后的细胞世代中持续存在的稳定结构。
