Mousa Sarah S, Mousa Shaymaa S, Mousa Shaker A
Pharmaceutical Research Institute at Albany, NY 12208, USA.
Nutr Cancer. 2005;52(1):59-65. doi: 10.1207/s15327914nc5201_8.
We carried out this investigation to examine the effects on angiogenesis-mediated processes and to define anti-angiogenesis mechanisms for flavonoids. We examined the effects and mechanisms of the flavonoid resveratrol on angiogenesis and tumor growth using the chick chorioallantoic membrane (CAM) model of angiogenesis, the CAM tumor growth model, and the effect on p53 in fibroblast growth factor-2 (FGF2) stimulated human endothelial cells using immunoassay. Resveratrol demonstrated potent inhibition (effective dose50=0.7+/-0.1 microM) of FGF2-induced angiogenesis and tumor growth. Furthermore, resveratrol significantly (P<0.01) inhibited platelet/fibrin clot-promoted human colon and fibrosarcoma tumor growth in the CAM tumor model. Resveratrol in a concentration-dependent (1-3 microM) manner significantly promoted apoptosis in FGF2-stimulated endothelial cells by increasing p53 protein production. These data indicated potent anti-angiogenesis efficacy, inhibition of tumor growth, and clot-mediated enhanced tumor growth. These data suggest potential anticancer benefits as a chemopreventive and chemotherapeutic for the flavonoid resveratrol.
我们开展这项研究以检测对血管生成介导过程的影响，并确定黄酮类化合物的抗血管生成机制。我们使用鸡胚绒毛尿囊膜（CAM）血管生成模型、CAM肿瘤生长模型，以及通过免疫测定法检测白藜芦醇对成纤维细胞生长因子-2（FGF2）刺激的人内皮细胞中p53的影响，来研究黄酮类化合物白藜芦醇对血管生成和肿瘤生长的作用及机制。白藜芦醇对FGF2诱导的血管生成和肿瘤生长表现出强效抑制作用（半数有效剂量=0.7±0.1微摩尔）。此外，在CAM肿瘤模型中，白藜芦醇显著（P<0.01）抑制血小板/纤维蛋白凝块促进的人结肠癌和纤维肉瘤肿瘤生长。白藜芦醇以浓度依赖方式（1-3微摩尔）通过增加p53蛋白生成，显著促进FGF2刺激的内皮细胞凋亡。这些数据表明其具有强效抗血管生成功效、抑制肿瘤生长以及凝块介导的肿瘤生长增强作用。这些数据提示黄酮类化合物白藜芦醇作为化学预防剂和化疗药物可能具有抗癌益处。
