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在一例角膜炎病例诊断中对阿利什霉DNA进行直接测序。

Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis.

作者信息

Mancini Nicasio, Ossi Cristina M, Perotti Mario, Carletti Silvia, Gianni Claudia, Paganoni Giorgio, Matuska Stanislav, Guglielminetti Maria, Cavallero Annalisa, Burioni Roberto, Rama Paolo, Clementi Massimo

机构信息

Laboratorio di Microbiologia, Diagnostica e Ricerca San Raffaele1 and Centro Malattie della Cornea, Dipartimento di Oftalmologia e Scienze della Visione3, IRCCS Istituto Scientifico San Raffaele, via Olgettina 60, 20132 Milano, Italy 2Università ''Vita-Salute San Raffaele'', Facoltà di Medicina e Chirurgia, Milano, Italy 4Dipartimento di Ecologia del Territorio e degli Ambienti Terrestri, Sezione di Micologia, Università degli Studi di Pavia, Pavia, Italy.

出版信息

J Med Microbiol. 2005 Sep;54(Pt 9):897-900. doi: 10.1099/jmm.0.46029-0.

DOI:10.1099/jmm.0.46029-0
PMID:16091444
Abstract

The present report describes the diagnostic strategy followed in a case of keratomycosis. Together with conventional methods, a molecular strategy that involved the direct sequencing of an amplified portion of the genome encompassing the internal transcribed spacer 1 and 2 regions and sequence analysis was used. The data highlight the diagnostic role of molecular techniques, in parallel with conventional methods, in the management of ocular infections of fungal aetiology.

摘要

本报告描述了在一例角膜真菌病病例中采用的诊断策略。除了传统方法外,还使用了一种分子策略,该策略涉及对包含内部转录间隔区1和2区域的基因组扩增部分进行直接测序及序列分析。这些数据突出了分子技术与传统方法并行在真菌性眼部感染管理中的诊断作用。

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