Ivanov Volodymyr, Wang Xiao-Hui, Tay Stephen Tiong-Lee, Tay Joo-Hwa
School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
Appl Microbiol Biotechnol. 2006 Apr;70(3):374-81. doi: 10.1007/s00253-005-0088-5. Epub 2005 Aug 10.
Microbial aggregates of an aerobic granular sludge can be used for the treatment of industrial or municipal wastewater, but their formation from a microbial activated sludge requires several weeks. Therefore, the aim of this research was the selection of microbial cultures to shorten the granule-forming period from several weeks to a few days. An enrichment culture with the ability to accelerate granulation was obtained by repeating the selection and batch cultivation of fast-settling microbial aggregates isolated from the aerobic granular sludge. Bacterial cultures of Klebsiella pneumoniae strain B and Pseudomonas veronii strain F, with self-aggregation indexes of 65 and 51%, respectively, and a coaggregation index of 58%, were isolated from the enrichment culture. A mixture of these strains with the activated sludge was used as an inoculum in an experimental sequencing batch reactor to start up an aerobic granulation process. Aerobic granules with a mean diameter of 446+/-76 microm were formed in an experiment after 8 days of cultivation, but microbial granules were absent in controls. Considering biosafety issues, K. pneumoniae strain B was excluded from further studies, but P. veronii strain F was selected for larger-scale testing.
好氧颗粒污泥的微生物聚集体可用于处理工业或城市废水，但其从微生物活性污泥中形成需要数周时间。因此，本研究的目的是选择微生物培养物，将颗粒形成期从数周缩短至几天。通过重复从好氧颗粒污泥中分离出的快速沉降微生物聚集体的选择和分批培养，获得了具有加速造粒能力的富集培养物。从富集培养物中分离出肺炎克雷伯菌菌株B和维罗纳假单胞菌菌株F的细菌培养物，其自聚集指数分别为65%和51%，共聚集指数为58%。这些菌株与活性污泥的混合物用作实验序批式反应器中的接种物，以启动好氧造粒过程。培养8天后，实验中形成了平均直径为446±76微米的好氧颗粒，但对照中没有微生物颗粒。考虑到生物安全问题，肺炎克雷伯菌菌株B被排除在进一步研究之外，但维罗纳假单胞菌菌株F被选用于更大规模的测试。
