Tremblay André J, Bergeron Jean, Gagné Jean-Marc, Gagné Claude, Couture Patrick
Lipid Research Center, CHUL Research Center, Quebec, Qc, Canada G1V 4G2.
Metabolism. 2005 Aug;54(8):1014-9. doi: 10.1016/j.metabol.2005.03.003.
Lipoprotein data and apolipoprotein (apo) E genotype from 1302 participants, covering a wide range of total plasma cholesterol levels, were used to examine the impact of apo E genotype on the estimation of low-density lipoprotein cholesterol (LDL-C0 concentrations by the Friedewald formula using high-density lipoprotein cholesterol and triglyceride (TG) concentrations as compared with the beta -quantification reference procedure. The results showed that participants with apo E2/E2 genotype had significantly higher very low-density lipoprotein cholesterol (VLDL-C) concentrations and VLDL-C/TG ratio as well as lower LDL-C concentrations than participants with other apo E genotypes. Heterozygous carriers of the epsilon 2 allele had significantly higher VLDL-C than participants with apo E3/E3 and E4/E3 genotypes. The mean absolute error and the mean percentage of bias in calculated LDL-C according to all apo E genotypes, except E2/E2 genotype, were less than 0.16 mmol/L and 4.4%, respectively. Indeed, the mean error and the mean percentage of bias associated with the LDL-C calculated by the Friedewald formula in the apo E2/E2 group were 0.93 mmol/L and 40.6%, respectively. However, participants with the apo E2/E2 genotype and a type III phenotype showed a mean error and a mean percentage of bias reaching 1.53 mmol/L and 63.5%, respectively, whereas E2/E2 participants with a non-type III phenotype had a mean error and a mean percentage of bias of 0.18 mmol/L and 11.0%, respectively. Moreover, 41.9% to 57.1% of the participants had an absolute bias higher than 5% according to the apo E genotype, except for the apo E2/E2 genotypic group where 88.6% of the participants had an absolute bias higher than 5%. Stepwise multiple linear regression analyses revealed that the apo E genotype contributed to 39.0% of the VLDL-C/TG ratio variance, whereas sex, age, and high-density lipoprotein cholesterol explained between 0.5% and 3.2% of the variance. These results indicate that the apo E genotype exerts a significant influence on the estimation of LDL-C concentrations by the Friedewald formula as compared with the beta-quantification.
对1302名参与者的脂蛋白数据和载脂蛋白（apo）E基因型进行了研究，这些参与者的血浆总胆固醇水平范围广泛。研究使用高密度脂蛋白胆固醇和甘油三酯（TG）浓度，通过Friedewald公式来检验apo E基因型对低密度脂蛋白胆固醇（LDL-C0）浓度估计的影响，并与β-定量参考程序进行比较。结果显示，与其他apo E基因型的参与者相比，apo E2/E2基因型的参与者极低密度脂蛋白胆固醇（VLDL-C）浓度和VLDL-C/TG比值显著更高，而LDL-C浓度更低。ε2等位基因的杂合携带者的VLDL-C显著高于apo E3/E3和E4/E3基因型的参与者。除E2/E2基因型外，根据所有apo E基因型计算的LDL-C的平均绝对误差和平均偏差百分比分别小于0.16 mmol/L和4.4%。事实上，apo E2/E2组中由Friedewald公式计算的LDL-C的平均误差和平均偏差百分比分别为0.93 mmol/L和40.6%。然而，具有apo E2/E2基因型和III型表型的参与者的平均误差和平均偏差百分比分别达到1.53 mmol/L和63.5%，而具有非III型表型的E2/E2参与者的平均误差和平均偏差百分比分别为0.18 mmol/L和11.0%。此外，根据apo E基因型，41.9%至57.1%的参与者的绝对偏差高于5%，而在apo E2/E2基因型组中，88.6%的参与者的绝对偏差高于5%。逐步多元线性回归分析显示，apo E基因型对VLDL-C/TG比值方差的贡献率为39.0%，而性别、年龄和高密度脂蛋白胆固醇对方差的解释率在0.5%至3.2%之间。这些结果表明，与β-定量相比，apo E基因型对Friedewald公式估算LDL-C浓度有显著影响。
