Jarzebiński J, Kratochwil W, Kwiatkowska B
Zakład Analizy Leków, Instytut Nauki o Leku, Akademia Medyczna, ul. Banacha 1, 02-097 Warszawa.
Acta Pol Pharm. 1992;49(5-6):5-9.
The term of isolation of arylosulfonic acids as hydrolysing factors from casein's hydrolyzates have been worked out. The most satisfactory results have been achieved when using in this case chloride 2,3,5-triphenylo-2H-tetrazole. When using benzenosulfonic acid as the best hydrolising factor 98.4% of this acid was removed from hydrolysates of hoofs whereas 96.1% was removed from hydrolysates of bristles. The residues of acids remaining in hydrolysates have been removed by shaking with Wofatyt SBW in form H+. The total recovery of aminoacids irrespectively to kind of hydrolyzate and applied acid amounts to 95.5% average.
已经确定了从酪蛋白水解物中分离出作为水解因子的芳基磺酸的条件。在这种情况下使用2,3,5-三苯基-2H-四唑氯化物时，取得了最令人满意的结果。当使用苯磺酸作为最佳水解因子时，从蹄角水解物中去除了98.4%的这种酸，而从猪鬃水解物中去除了96.1%。通过用H+形式的Wofatyt SBW振荡，去除了水解物中残留的酸。无论水解物的种类和所用酸的量如何，氨基酸的总回收率平均为95.5%。
