血红蛋白结构和变构变化自由能的氢交换测量。
Hydrogen exchange measurement of the free energy of structural and allosteric change in hemoglobin.
作者信息
Englander S W, Englander J J, McKinnie R E, Ackers G K, Turner G J, Westrick J A, Gill S J
机构信息
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104-6059.
出版信息
Science. 1992 Jun 19;256(5064):1684-7. doi: 10.1126/science.256.5064.1684.
Abstract
The inability to localize and measure the free energy of protein structure and structure change severely limits protein structure-function investigations. The local unfolding model for protein hydrogen exchange quantitatively related the free energy of local structural stability with the hydrogen exchange rate of concerted sets of structurally related protons. In tests with a number of modified hemoglobin forms, the loss in structural free energy obtained locally from hydrogen exchange results matches the loss in allosteric free energy measured globally by oxygen-binding and subunit dissociation experiments.
摘要
无法定位和测量蛋白质结构及其变化的自由能，这严重限制了蛋白质结构与功能的研究。蛋白质氢交换的局部去折叠模型将局部结构稳定性的自由能与结构相关质子协同组的氢交换速率定量关联起来。在对多种修饰血红蛋白形式的测试中，通过氢交换结果局部获得的结构自由能损失与通过氧结合和亚基解离实验全局测量的变构自由能损失相匹配。
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本文引用的文献
1
LINKED FUNCTIONS AND RECIPROCAL EFFECTS IN HEMOGLOBIN: A SECOND LOOK.血红蛋白中的关联功能与相互作用：再审视
Adv Protein Chem. 1964;19:223-86. doi: 10.1016/s0065-3233(08)60190-4.
2
Deuterium exchange of poly-DL-alanine in aqueous solution.聚-DL-丙氨酸在水溶液中的氘交换
Arch Biochem Biophys. 1957 Jul;69:106-18. doi: 10.1016/0003-9861(57)90478-2.
3
The slowest allosterically responsive hydrogens in hemoglobin. Completion of the hydrogen exchange survey.
J Biol Chem. 1980 Nov 25;255(22):10695-701.
4
Individual breathing reactions measured in hemoglobin by hydrogen exchange methods.通过氢交换法在血红蛋白中测量的个体呼吸反应。
Biophys J. 1980 Oct;32(1):577-89. doi: 10.1016/S0006-3495(80)84991-5.
5
Hydrogen exchange studies of proteins: recent advances in medium and high resolution methods.蛋白质的氢交换研究：中高分辨率方法的最新进展
J Biochem Biophys Methods. 1983 Jul;7(4):345-57. doi: 10.1016/0165-022x(83)90060-x.
6
Identification of an allosterically sensitive unfolding unit in hemoglobin.血红蛋白中变构敏感解折叠单元的鉴定。
J Mol Biol. 1983 Sep 5;169(1):325-44. doi: 10.1016/s0022-2836(83)80186-7.
7
Exchange behavior of the H-bonded amide protons in the 3 to 13 helix of ribonuclease S.核糖核酸酶S的3至13螺旋中氢键结合酰胺质子的交换行为。
J Mol Biol. 1983 Sep 5;169(1):299-323. doi: 10.1016/s0022-2836(83)80185-5.
8
Amide protein exchange and surface conformation of the basic pancreatic trypsin inhibitor in solution. Studies with two-dimensional nuclear magnetic resonance.溶液中碱性胰蛋白酶抑制剂的酰胺蛋白交换与表面构象。二维核磁共振研究。
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9
Comparison of experimental binding data and theoretical models in proteins containing subunits.含亚基蛋白质中实验结合数据与理论模型的比较。
Biochemistry. 1966 Jan;5(1):365-85. doi: 10.1021/bi00865a047.
10
Stereochemistry of cooperative effects in haemoglobin.血红蛋白协同效应的立体化学
Nature. 1970 Nov 21;228(5273):726-39. doi: 10.1038/228726a0.
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