Tilquin Pierre, Barrow Paul A, Marly José, Pitel Frédérique, Plisson-Petit Florence, Velge Philippe, Vignal Alain, Baret Philippe V, Bumstead Nat, Beaumont Catherine
Unité de Génétique, Faculté d'ingénierie biologique, agronomique et environnementale, Université catholique de Louvain, Croix du Sud 2 bte 14, B-1348 Louvain-la-Neuve, Belgium.
Genet Sel Evol. 2005 Sep-Oct;37(5):539-61. doi: 10.1186/1297-9686-37-6-539.
Selection for increased resistance to Salmonella colonisation and excretion could reduce the risk of foodborne Salmonella infection. In order to identify potential loci affecting resistance, differences in resistance were identified between the N and 6(1) inbred lines and two QTL research performed. In an F2 cross, the animals were inoculated at one week of age with Salmonella enteritidis and cloacal swabs were carried out 4 and 5 wk post inoculation (thereafter called CSW4F2 and CSW4F2) and caecal contamination (CAECF2) was assessed 1 week later. The animals from the (N x 6(1)) x N backcross were inoculated at six weeks of age with Salmonella typhimurium and cloacal swabs were studied from wk 1 to 4 (thereafter called CSW1BC to CSW4BC). A total of 33 F2 and 46 backcross progeny were selectively genotyped for 103 and 135 microsatellite markers respectively. The analysis used least-squares-based and non-parametric interval mapping. Two genome-wise significant QTL were observed on Chromosome 1 for CSW2BC and on Chromosome 2 for CSW4F2, and four suggestive QTL for CSW5F2 on Chromosome 2, for CSW5F2 and CSW2BC on chromosome 5 and for CAECF2 on chromosome 16. These results suggest new regions of interest and the putative role of SAL1.
选择增强对沙门氏菌定植和排泄的抵抗力可降低食源性沙门氏菌感染的风险。为了确定影响抵抗力的潜在基因座，鉴定了N和6(1)近交系之间的抵抗力差异，并进行了两项QTL研究。在一个F2杂交群体中，动物在1周龄时接种肠炎沙门氏菌，并在接种后4周和5周进行泄殖腔拭子采集（此后称为CSW4F2和CSW5F2），1周后评估盲肠污染情况（CAECF2）。(N×6(1))×N回交群体的动物在6周龄时接种鼠伤寒沙门氏菌，并对第1周~4周的泄殖腔拭子进行研究（此后称为CSW1BC至CSW4BC）。分别对33个F2和46个回交后代进行了选择性基因分型，分别针对103个和135个微卫星标记。分析采用基于最小二乘法和非参数区间作图法。在第1号染色体上观察到两个全基因组显著的QTL，分别对应CSW2BC和CSW4F2；在第2号染色体上观察到四个提示性QTL，分别对应CSW5F2、在第5号染色体上对应CSW5F2和CSW2BC以及在第16号染色体上对应CAECF2。这些结果提示了新的感兴趣区域以及SAL1的假定作用。
