Penedo M C T, Millon L V, Bernoco D, Bailey E, Binns M, Cholewinski G, Ellis N, Flynn J, Gralak B, Guthrie A, Hasegawa T, Lindgren G, Lyons L A, Røed K H, Swinburne J E, Tozaki T
School of Veterinary Medicine, University of California, Davis, CA, USA.
Cytogenet Genome Res. 2005;111(1):5-15. doi: 10.1159/000085664.
A comprehensive male linkage map was generated by adding 359 new, informative microsatellites to the International Equine Gene Map half-sibling reference families and by combining genotype data from three independent mapping resources: a full sibling family created at the Animal Health Trust in Newmarket, United Kingdom, eight half-sibling families from Sweden and two half-sibling families from the University of California, Davis. Because the combined data were derived primarily from half-sibling families, only autosomal markers were analyzed. The map was constructed from a total of 766 markers distributed on the 31 equine chromosomes. It has a higher marker density than that of previously reported maps, with 626 markers linearly ordered and 140 other markers assigned to a chromosomal region. Fifty-nine markers (7%) failed to meet the criteria for statistical evidence of linkage and remain unassigned. The map spans 3,740 cM with an average distance of 6.3 cM between markers. Fifty-five percent of the intervals are < or = 5 cM and only 3% > or = 20 cM. The present map demonstrates the cohesiveness of the different data sets and provides a single resource for genome scan analyses and integration with the radiation hybrid map.
通过将359个新的、信息丰富的微卫星添加到国际马基因图谱半同胞参考家系中，并结合来自三个独立定位资源的基因型数据，构建了一个全面的雄性连锁图谱：一个在英国纽马克特动物健康信托中心创建的全同胞家系、来自瑞典的八个半同胞家系以及来自加利福尼亚大学戴维斯分校的两个半同胞家系。由于合并的数据主要来自半同胞家系，因此仅分析常染色体标记。该图谱由分布在31条马染色体上的总共766个标记构建而成。它的标记密度高于先前报道的图谱，有626个标记线性排列，另外140个标记被分配到一个染色体区域。59个标记（7%）未达到连锁统计证据的标准，仍未分配。该图谱跨度为3740厘摩，标记之间的平均距离为6.3厘摩。55%的区间小于或等于5厘摩，只有3%大于或等于20厘摩。本图谱展示了不同数据集的凝聚性，并为基因组扫描分析以及与辐射杂种图谱的整合提供了单一资源。
