Kaji T, Kawashima T, Yamamoto C, Sakamoto M
Department of Environmental Science, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Toxicol Lett. 1992 Jun;61(1):81-7. doi: 10.1016/0378-4274(92)90066-s.
To investigate the effect of the cosmetic dye, rhodamine B, on the metabolism of collagen in fibroblasts, confluent KD cells, an established cell line of fibroblasts from human lip, were cultured for 6 h in a serum-free medium in the presence of the dye at 100 micrograms/ml and below. It was found that rhodamine B significantly decreased the content of procollagen type I C-terminal peptide antigen in both the cell layer and the medium with an only slight decrease in the cell number. Rhodamine B significantly decreased the incorporation of [3H]proline into either the collagen-digestible protein or the non-collagen protein in the cell layer. The incorporation of both [3H]thymidine and [14C]leucine into the acid-insoluble fraction of the cell layer was significantly decreased by rhodamine B; the activity of lactate dehydrogenase that leaked into the medium was not changed by the dye. From these results, it was suggested that rhodamine B has the capacity of decreasing the collagen content of the fibroblast cell layer of the human lip, which may result from a non-specific inhibition of protein synthesis without non-specific cell damage. Rhodamine B may impair the formation of extracellular matrix which is important for the maintenance of the lip tissue.
为研究化妆品染料罗丹明B对成纤维细胞中胶原蛋白代谢的影响，将人唇部成纤维细胞系KD细胞在无血清培养基中，于100微克/毫升及以下浓度的染料存在下培养6小时。结果发现，罗丹明B显著降低了细胞层和培养基中I型前胶原C端肽抗原的含量，而细胞数量仅有轻微减少。罗丹明B显著降低了[3H]脯氨酸掺入细胞层中可被胶原酶消化的蛋白质或非胶原蛋白中的量。罗丹明B显著降低了[3H]胸腺嘧啶核苷和[14C]亮氨酸掺入细胞层酸不溶性部分的量；染料对泄漏到培养基中的乳酸脱氢酶活性没有影响。从这些结果推测，罗丹明B具有降低人唇部成纤维细胞层胶原蛋白含量的能力，这可能是由于对蛋白质合成的非特异性抑制，而无细胞非特异性损伤。罗丹明B可能损害对维持唇部组织很重要的细胞外基质的形成。
