Zhang Qi-Jun, Ye Shao-Ping, Li Jie-Qin, Zhao Bing, Liang Yong-Shu, Peng Yong, Li Ping
Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China.
Yi Chuan Xue Bao. 2006 Feb;33(2):152-60. doi: 10.1016/S0379-4172(06)60034-6.
Based on the successful development of new microsatellite markers from the data of two whole-sequenced rice varieties, japonica variety Nipponbare and indica variety 9311, an F2 population of 90 lines, which was derived from a single cross between Nipponbare and 9311, was applied to construct a genetic linkage framework map. The map covered 2 455.7 cM of total genomic length, and consisted of 152 simple sequence repeats (SSRs) loci including 46 pairs of new SSR primers developed by our research institute. The average genetic distance between two markers was 16.16 cM. In addition, markers RM345 and RM494, which have not been mapped on the Temnykh's map et al. (2001) were anchored on the sixth chromosome of this map. We compared this research with maps of Temnykh et al.(2001) and LAN et al. (2003) regarding the aspects of type and size of population, type and quantity of markers, and the marker arrangement order on chromosome, etc. Results indicated that the similarity of marker linear alignment was 93.81% between this map and T-map. Finally, the important significance of using sequenced rice varieties to construct linkage map was also discussed.
基于从两个全基因组测序水稻品种（粳稻品种日本晴和籼稻品种9311）的数据成功开发出新的微卫星标记，一个由90个株系组成的F2群体（该群体源自日本晴和9311的单交组合）被用于构建遗传连锁框架图谱。该图谱覆盖的基因组总长度为2455.7厘摩，由152个简单序列重复（SSR）位点组成，其中包括由本研究所开发的46对新SSR引物。两个标记之间的平均遗传距离为16.16厘摩。此外，尚未定位在Temnykh等人（2001年）图谱上的标记RM345和RM494被定位在该图谱的第六条染色体上。我们在群体类型和大小、标记类型和数量以及染色体上标记排列顺序等方面，将本研究与Temnykh等人（2001年）和LAN等人（2003年）的图谱进行了比较。结果表明，该图谱与T图谱之间标记线性排列的相似性为93.81%。最后，还讨论了利用测序水稻品种构建连锁图谱的重要意义。
