Godinho Kevin S, Keane Sue G, Nanjiani Ian A, Benchaoui Hafid A, Sunderland Simon J, Jones M Anne, Weatherley Andrew J, Gootz Thomas D, Rowan Tim G
Veterinary Medicine Research and Development, Pfizer Ltd, Sandwich, Kent, UK.
Vet Ther. 2005 Summer;6(2):113-21.
The in vitro activity of tulathromycin was evaluated against common bovine and porcine respiratory pathogens collected from outbreaks of clinical disease across eight European countries from 1998 to 2001. Minimum inhibitory concentrations (MICs) for one isolate of each bacterial species from each outbreak were determined using a broth microdilution technique. The lowest concentrations inhibiting the growth of 90% of isolates (MIC90) for tulathromycin were 2 microg/ml for Mannheimia (Pasteurella) haemolytica, 1 microg/ml for Pasteurella multocida (bovine), and 2 microg/ml for Pasteurella multocida (porcine) and ranged from 0.5 to 4 microg/ml for Histophilus somni (Haemophilus somnus) and from 4 to 16 microg/ml for Actinobacillus pleuropneumoniae. Isolates were retested in the presence of serum. The activity of tulathromycin against fastidious organisms was affected by culture conditions, and MICs were reduced in the presence of serum.
对1998年至2001年从八个欧洲国家临床疾病暴发中收集的常见牛和猪呼吸道病原体评估了泰拉霉素的体外活性。使用肉汤微量稀释技术确定每次暴发中每种细菌分离株的最低抑菌浓度(MIC)。泰拉霉素对90%分离株的最低抑菌浓度(MIC90),溶血曼氏杆菌(巴斯德氏菌属)为2微克/毫升,多杀巴斯德氏菌(牛)为1微克/毫升,多杀巴斯德氏菌(猪)为2微克/毫升,睡眠嗜组织菌(睡眠嗜血杆菌)为0.5至4微克/毫升,胸膜肺炎放线杆菌为4至16微克/毫升。在有血清存在的情况下对分离株进行重新测试。泰拉霉素对苛求菌的活性受培养条件影响,在有血清存在时MIC降低。