Bertini Ivano, Luchinat Claudio, Parigi Giacomo, Pierattelli Roberta
Magnetic Resonance Center, University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy.
Chembiochem. 2005 Sep;6(9):1536-49. doi: 10.1002/cbic.200500124.
This article deals with the solution structure determination of paramagnetic metalloproteins by NMR spectroscopy. These proteins were believed not to be suitable for NMR investigations for structure determination until a decade ago, but eventually novel experiments and software protocols were developed, with the aim of making the approach suitable for the goal and as user-friendly and safe as possible. In the article, we also give hints for the optimization of experiments with respect to each particular metal ion, with the aim of also providing a handy tool for nonspecialists. Finally, a section is dedicated to the significant progress made on 13C direct detection, which reduces the negative effects of paramagnetism and may constitute a new chapter in the whole field of NMR spectroscopy.
本文论述了利用核磁共振光谱法测定顺磁性金属蛋白的溶液结构。直到十年前，人们还认为这些蛋白质不适合用于核磁共振结构测定研究，但最终开发出了新的实验和软件协议，目的是使该方法适用于此目标，并尽可能方便用户且安全。在本文中，我们还针对每种特定金属离子给出了实验优化的提示，目的是为非专业人员提供一个便捷工具。最后，有一部分专门介绍了在13C直接检测方面取得的重大进展，该进展减少了顺磁性的负面影响，可能会在核磁共振光谱学的整个领域开启新的篇章。
