Delgado-Lista J, Perez-Jimenez F, Gavilan E, Marin C, Fuentes F, Fernandez-Puebla R A, Perez-Martinez P, Paniagua J A, Aguilera C, Lopez-Miranda J
Lipid and Arteriosclerosis Research Unit, Department of Medicine, Hospital Universitario Reina Sofía, School of Medicine, University of Córdoba, 14004 Córdoba, Spain.
Lipids. 2005 May;40(5):471-6. doi: 10.1007/s11745-005-1406-0.
Paraoxonase 1 (PON 1) is an esterase with antioxidant properties that is present in HDL. Gln192Arg polymorphism (also named Q192R or Q/R) of the PON 1 gene that encodes this protein defines two alleles (Q and R). The R allele has been associated with higher cardiovascular risk. LDL size and susceptibility to oxidation also have been identified as cardiovascular risk factors. Our objective was to determine whether genetic variations in the Gln192Arg polymorphism influence LDL size and susceptibility to oxidation after the consumption of diets with different fat content. In our experiments, the participants (n = 98) underwent three 4-wk diets--one, saturated fat-enriched (SAT); another, monounsaturated fat-enriched (MONO); and a third, carbohydrate-enriched (CHO). We observed that LDL were smaller in the QQ group after the CHO diet vs. the SAT (P < 0.01) and MONO diets (P < 0.03). No differences in LDL size were found in QR/RR subjects. When we analyzed lag time of oxidation of LDL, we found that when carriers of the R allele (QR/RR) received the MONO diet, the lag period of LDL oxidation was longer as compared with the CHO diet. Otherwise, we found no differences in QQ homozygotes when we evaluated the lag time of oxidation of LDL after the three diets. These results suggest that the Glnl92Arg polymorphism of the paraoxonase gene influences LDL size and susceptibility to oxidation in response to diet.
对氧磷酶1（PON 1）是一种具有抗氧化特性的酯酶，存在于高密度脂蛋白（HDL）中。编码该蛋白的PON 1基因的Gln192Arg多态性（也称为Q192R或Q/R）定义了两个等位基因（Q和R）。R等位基因与较高的心血管风险相关。低密度脂蛋白（LDL）大小和氧化易感性也已被确定为心血管危险因素。我们的目的是确定Gln192Arg多态性的基因变异在摄入不同脂肪含量的饮食后是否会影响LDL大小和氧化易感性。在我们的实验中，参与者（n = 98）接受了三种为期4周的饮食——一种富含饱和脂肪（SAT）；另一种富含单不饱和脂肪（MONO）；第三种富含碳水化合物（CHO）。我们观察到，与SAT饮食（P < 0.01）和MONO饮食（P < 0.03）相比，CHO饮食后QQ组的LDL较小。QR/RR受试者的LDL大小没有差异。当我们分析LDL氧化的延迟时间时，我们发现，当R等位基因携带者（QR/RR）接受MONO饮食时，与CHO饮食相比，LDL氧化的延迟期更长。否则，当我们评估三种饮食后LDL氧化的延迟时间时，我们发现QQ纯合子没有差异。这些结果表明，对氧磷酶基因的Gln192Arg多态性会影响LDL大小以及饮食引起的氧化易感性。
