Pouwels P H, van Rotterdam J
Medical Biological Laboratory TNO, Rijswijk Z. H., The Netherlands.
Mol Gen Genet. 1975;136(3):215-26. doi: 10.1007/BF00334016.
A protein fraction, called At (= anti termination) factor, has been isolated from extracts of E. coli and partially purified. The At factor stimulates the synthesis in vitro of anthranilate synthetase, an enzyme encoded by two genes of the tryptophan (trp) operon, but has no effect on the synthesis of T7 RNA polymerase and other T7- and T4 coded proteins. The At factor stimulates the synthesis of trp mRNA; it has no effect on the translation of trp mRNA. We conclude that in vitro transcription of the trp operon is positively controlled.
一种名为At（抗终止）因子的蛋白质组分已从大肠杆菌提取物中分离出来并进行了部分纯化。At因子刺激体外邻氨基苯甲酸合成酶的合成，邻氨基苯甲酸合成酶是一种由色氨酸（trp）操纵子的两个基因编码的酶，但对T7 RNA聚合酶和其他T7及T4编码蛋白的合成没有影响。At因子刺激trp mRNA的合成；它对trp mRNA的翻译没有影响。我们得出结论，trp操纵子的体外转录受到正调控。
