Davison J, Pilarski L M, Echols H
Proc Natl Acad Sci U S A. 1969 May;63(1):168-74. doi: 10.1073/pnas.63.1.168.
A protein factor, partially purified from E. coli, can stimulate some 30- to 50-fold the transcription of DNA by purified RNA polymerase. Evidence is presented suggesting that this stimulatory factor (designated M factor) exerts its effect before the RNA synthesis is initiated. The M factor is prepared from a crude ribosome fraction, but it does not require the presence of ribosomes for full activity.
一种从大肠杆菌中部分纯化得到的蛋白质因子,可使纯化的RNA聚合酶对DNA的转录活性提高约30至50倍。有证据表明,这种刺激因子(称为M因子)在RNA合成起始之前就发挥作用。M因子是从粗核糖体组分中制备的,但它的充分活性并不需要核糖体的存在。