Palumbo Maria Concetta, Colosimo Alfredo, Giuliani Alessandro, Farina Lorenzo
Physiology and Pharmacology Department, University of Rome, La Sapienza, Rome, Italy.
FEBS Lett. 2005 Aug 29;579(21):4642-6. doi: 10.1016/j.febslet.2005.07.033.
The relation between the position of mutations in Saccharomyces cerevisiae metabolic network and their lethality is the subject of this work. We represent the topology of the network by a directed graph: nodes are metabolites and arcs represent the reactions; a mutation corresponds to the removal of all the arcs referring to the deleted enzyme. Using publicly available knock-out data, we show that lethality corresponds to the lack of alternative paths in the perturbed network linking the nodes affected by the enzyme deletion. Such feature is at the basis of the recently recognized importance of 'marginal' arcs of metabolic networks.
酿酒酵母代谢网络中突变位置与其致死性之间的关系是本研究的主题。我们用有向图来表示网络拓扑结构：节点是代谢物，弧表示反应；一个突变对应于删除所有与被删除酶相关的弧。利用公开可用的基因敲除数据，我们表明致死性对应于受酶缺失影响的节点在受干扰网络中缺乏替代路径。这一特征是代谢网络中“边缘”弧最近被认识到的重要性的基础。
