Li Guangke, Sang Nan, Wang Qian
College of Environment and Resource, Shanxi University, Taiyuan, Shanxi 030006, People's Republic of China.
Ecotoxicol Environ Saf. 2006 Sep;65(1):134-9. doi: 10.1016/j.ecoenv.2005.06.011. Epub 2005 Aug 10.
The effects of the Xingou municipal landfill leachate on levels of thiobarbituric acid reactive substances (TBARS) and the activities of Cu,Zn-superoxide dismutase (Cu,Zn-SOD), Se-dependent glutathione peroxidase (Se-dependent GPx), and catalase (CAT) were investigated in brains and livers of Kunming albino mice of both sexes, using chemical oxygen demand (COD(Cr)) as a measure of leachate concentration. The results show that leachate caused lipid peroxidation and change of antioxidative status in brains and livers of mice. There was a sex difference in the response of antioxidative status, and female mice were more sensitive than male mice. Exposure to leachate at all concentrations tested caused significant increases of TBARS levels in brains and livers from mice of both sexes. For brains, Cu,Zn-SOD and Se-dependent GPx activities were significantly increased at high concentration (COD(Cr) 240 mg/L) for male mice, but the activities of both antioxidative enzymes were significantly increased at low concentration (COD(Cr) 60 mg/L) and decreased at high concentration (COD(Cr) 240 mg/L) for female mice; CAT activities remained unchanged for male mice and were significantly decreased for female mice at high concentration (COD(Cr) 240 mg/L). For livers, Cu,Zn-SOD and Se-dependent GPx activities were significantly increased at high concentrations (COD(Cr) 120 and 240 mg/L) for male mice, but the activities of both antioxidative enzymes were significantly increased at low concentration (COD(Cr) 30 or 60 mg/L) and decreased at high concentration (COD(Cr) 240 mg/L) for female mice; CAT activities were significantly increased for male mice at all concentrations tested and decreased for female mice at high concentrations (COD(Cr) 120 and 240 mg/L). These results suggest that leachate exposure can cause oxidative damage on brains and livers of mice. The results also suggest that leachate might induce toxicity in mammals by the free-radical-damage mechanism.
以化学需氧量（COD(Cr)）作为渗滤液浓度的衡量指标，研究了兴沟市垃圾填埋场渗滤液对雌雄昆明白化小鼠脑和肝脏中硫代巴比妥酸反应性物质（TBARS）水平以及铜锌超氧化物歧化酶（Cu,Zn-SOD）、硒依赖性谷胱甘肽过氧化物酶（Se-依赖性GPx）和过氧化氢酶（CAT）活性的影响。结果表明，渗滤液导致小鼠脑和肝脏中的脂质过氧化以及抗氧化状态的改变。抗氧化状态的反应存在性别差异，雌性小鼠比雄性小鼠更敏感。在所有测试浓度下，暴露于渗滤液都会导致雌雄小鼠脑和肝脏中TBARS水平显著升高。对于脑，高浓度（COD(Cr) 240 mg/L）时雄性小鼠的Cu,Zn-SOD和Se-依赖性GPx活性显著增加，但低浓度（COD(Cr) 60 mg/L）时雌性小鼠的这两种抗氧化酶活性显著增加，高浓度（COD(Cr) 240 mg/L）时则降低；雄性小鼠的CAT活性保持不变，高浓度（COD(Cr) 240 mg/L）时雌性小鼠的CAT活性显著降低。对于肝脏，高浓度（COD(Cr) 120和240 mg/L）时雄性小鼠的Cu,Zn-SOD和Se-依赖性GPx活性显著增加，但低浓度（COD(Cr) 30或60 mg/L）时雌性小鼠的这两种抗氧化酶活性显著增加，高浓度（COD(Cr) 240 mg/L）时则降低；所有测试浓度下雄性小鼠的CAT活性均显著增加，高浓度（COD(Cr) 120和240 mg/L）时雌性小鼠的CAT活性降低。这些结果表明，暴露于渗滤液会对小鼠的脑和肝脏造成氧化损伤。结果还表明，渗滤液可能通过自由基损伤机制诱导哺乳动物中毒。
