Al-Dosari Mohammed S, Knapp Joseph E, Liu Dexi
Department of Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy, Pittsburgh, Pennsylvania 15261, USA.
Adv Genet. 2005;54:65-82. doi: 10.1016/S0065-2660(05)54004-5.
Hydrodynamic delivery has emerged as a near-perfect method for intracellular DNA delivery in vivo. For gene delivery to parenchymal cells, only essential DNA sequences need to be injected via a selected blood vessel, eliminating safety concerns associated with current viral and synthetic vectors. When injected into the bloodstream, DNA is capable of reaching cells in the different tissues accessible to the blood. Hydrodynamic delivery employs the force generated by the rapid injection of a large volume of solution into the incompressible blood in the circulation to overcome the physical barriers of endothelium and cell membranes that prevent large and membrane-impermeable compounds from entering parenchymal cells. In addition to the delivery of DNA, this method is useful for the efficient intracellular delivery of RNA, proteins, and other small compounds in vivo. This review discusses the development, current application, and clinical potential of hydrodynamic delivery.
流体动力学递送已成为一种近乎完美的体内细胞内DNA递送方法。对于将基因递送至实质细胞，仅需通过选定的血管注射必需的DNA序列，从而消除了与当前病毒和合成载体相关的安全隐患。当注入血液中时，DNA能够到达血液可及的不同组织中的细胞。流体动力学递送利用将大量溶液快速注入循环中不可压缩的血液所产生的力，来克服内皮和细胞膜的物理屏障，这些屏障会阻止大分子和膜不可渗透的化合物进入实质细胞。除了递送DNA外，该方法还可用于在体内高效地将RNA、蛋白质和其他小分子化合物递送至细胞内。本文综述了流体动力学递送的发展、当前应用及临床潜力。
