van der Plas A, Nijweide P J
Laboratory of Cell Biology and Histology, University of Leiden, The Netherlands.
J Bone Miner Res. 1992 Apr;7(4):389-96. doi: 10.1002/jbmr.5650070406.
An isolation method for osteocytes is described. After removal of the periostea, bone cells were isolated from calvariae of 18-day-old chicken embryos by alternating treatments with collagenase and EDTA. Osteocytes were purified from the heterogeneous bone cell population with the help of the osteocyte-specific MAb OB 7.3 bound to protein G-conjugated magnetic beads. The purity of the osteocyte population ultimately obtained was more than 95%. Osteocytes were found to adhere rapidly to glass or plastic substrates. They showed numerous processes of various types. These processes could branch and make contact with those of other osteocytes. After 1-2 days of culture, the isolated osteocytes formed a network of apparently interconnected cell processes very similar to the osteocyte network in bone.
本文描述了一种骨细胞的分离方法。去除骨膜后，通过胶原酶和乙二胺四乙酸（EDTA）交替处理，从18日龄鸡胚的颅骨中分离出骨细胞。借助与蛋白G偶联磁珠结合的骨细胞特异性单克隆抗体OB 7.3，从异质性骨细胞群体中纯化出骨细胞。最终获得的骨细胞群体纯度超过95%。发现骨细胞能迅速黏附于玻璃或塑料基质。它们呈现出多种类型的众多突起。这些突起可以分支并与其他骨细胞的突起接触。培养1 - 2天后，分离出的骨细胞形成了一个由明显相互连接的细胞突起组成的网络，与骨中的骨细胞网络非常相似。
