Harper G, Hart D, Moult S, Hull R, Geering A, Thomas J
Department of Disease and Stress Biology, John Innes Centre, Colney Lane, Norwich, UK.
Arch Virol. 2005 Dec;150(12):2407-20. doi: 10.1007/s00705-005-0610-1. Epub 2005 Aug 12.
In a study of the variation among isolates of Banana streak virus (BSV) in Uganda, 140 sequences were obtained from 49 samples by PCR across the conserved reverse transcriptase/RNaseH region of the genome. Pairwise comparison of these sequences suggested that they represented 15 different species and phylogenetic analyses showed that all species fell into three major clades based on 28% sequence difference. In addition to the Ugandan sequences, clade I also contained BSV species that are known as both integrated sequences and episomal viruses; clade II also contained integrated BSV sequences but which have not previously been identified as episomal viruses. Clade III comprised of Sugarcane bacilliform virus isolates and Ugandan BSV sequences and for which there is no evidence of integration. The possible reasons for the extraordinary levels of virus sequence variation and the potential origins and epidemiology of these viruses causing banana streak disease are discussed.
在一项关于乌干达香蕉条纹病毒（BSV）分离株变异的研究中，通过PCR扩增基因组保守的逆转录酶/核糖核酸酶H区域，从49个样本中获得了140个序列。这些序列的两两比较表明，它们代表了15个不同的物种，系统发育分析显示，基于28%的序列差异，所有物种可分为三个主要进化枝。除了乌干达的序列外，进化枝I还包含既作为整合序列又作为游离病毒存在的BSV物种；进化枝II也包含整合的BSV序列，但此前未被鉴定为游离病毒。进化枝III由甘蔗杆状病毒分离株和乌干达BSV序列组成，且没有整合的证据。文中讨论了病毒序列变异水平异常的可能原因以及这些引起香蕉条纹病的病毒的潜在起源和流行病学。
