Taib Mariam, Pinney John W, Westhead David R, McDowall Kenneth J, Adams David J
School of Biochemistry & Microbiology, University of Leeds, LS2 9JT, Leeds, UK.
Arch Microbiol. 2005 Oct;184(1):78-81. doi: 10.1007/s00203-005-0028-x. Epub 2005 Nov 3.
We provide the first indication of the extent of the complex chitinolytic system of a filamentous fungus. Phylogenetic analysis of the 14 apparent chitinases of the opportunistic fungal pathogen Aspergillus fumigatus identified four and ten enzymes related to plant and bacterial chitinases, respectively. Further, real time-RT-PCR studies revealed distinct patterns of gene expression, consistent with morphogenetic or nutritional roles, for members of the fungal/plant or fungal/bacterial sub-families, respectively. Our results provide a basis for future studies with A. fumigatus chitinases, which may lead to the exploitation of these enzymes, or their regulators, in the development of novel drug strategies.
我们首次揭示了丝状真菌复杂几丁质酶系统的范围。对机会性真菌病原体烟曲霉的14种表观几丁质酶进行系统发育分析，结果分别鉴定出4种与植物几丁质酶相关的酶和10种与细菌几丁质酶相关的酶。此外，实时逆转录聚合酶链反应（real time-RT-PCR）研究分别揭示了真菌/植物或真菌/细菌亚家族成员不同的基因表达模式，这与形态发生或营养作用一致。我们的研究结果为未来对烟曲霉几丁质酶的研究提供了基础，这可能会在新型药物策略的开发中促进对这些酶或其调节剂的利用。
