Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km2 (28220), Majadahonda, Madrid, Spain.
Fungal Genet Biol. 2011 Apr;48(4):418-29. doi: 10.1016/j.fgb.2010.12.007. Epub 2010 Dec 22.
A quintuple mutant was constructed to delete the entire family of the fungal/plant (class III) chitinases of Aspergillus fumigatus. Only a limited reduction in the total chitinolytic activity was seen for the different chitinase mutants including the quintuple mutant. In spite of this reduction in chitinolytic activity, no growth or germination defects were observed in these chitinase mutants. This result demonstrated that the fungal/plant chitinases do not have an essential role in the morphogenesis of A. fumigatus. A slight diminution of the growth during autolysis was seen for the quintuple mutant suggesting that class III chitinases may play only a nutritional role during this phase of the cycle, retarding fungal death.
构建了一个五重突变体来删除烟曲霉真菌/植物（III 类）几丁质酶的整个家族。不同的几丁质酶突变体，包括五重突变体，其总几丁质酶活性仅出现有限的降低。尽管几丁质酶活性降低，但这些几丁质酶突变体没有观察到生长或发芽缺陷。这一结果表明真菌/植物几丁质酶在烟曲霉的形态发生中没有重要作用。五重突变体在自溶过程中的生长略有减少，表明 III 类几丁质酶在这个周期阶段可能只发挥营养作用，延缓真菌死亡。
