Revelos Kyriakos, Petraki Constantina, Gregorakis Alkiviadis, Scorilas Andreas, Papanastasiou Panagiotis, Tenta Roxane, Koutsilieris Michael
Department of Pathology, "Evangelismos" General Hospital, Athens, Greece.
In Vivo. 2005 Sep-Oct;19(5):911-20.
The immunohistochemical expressions (IE) of p27(kip1) and Ki-67 (MIB-1), both involved in cell cycle regulation and cell proliferation, and their ability to predict biochemical failure, were assessed in patients with clinically localized prostate cancer who had underdone radical prostatectomy of curative intent. In addition, p27(kip1) and Ki-67 (MIB1) expressions were correlated with several pre-operative and post-operative parameters, such as Gleason score, extracapsular extension, seminal vesicle involvement, pelvic lymph nodes metastasis, positive surgical margins, coexistence of high-grade prostatic intraepithelial neoplasia, tumour size, prostate volume and PSA levels. Our analysis involved 130 consecutive radical prostatectomy specimens. A statistically significant correlation of low p27(kiP1) IE with seminal vesicles involvement, increased tumour volume and high pre-operative PSA values was documented. Low p27(kiP1) IE was significantly correlated with an increased likelihood of biochemical failure after radical prostatectomy. In addition, the increased IE of Ki-67 (MIB1) correlated significantly with metastatic disease in the pelvic lymph nodes and was a significant predictor of biochemical failure. Cox regression analysis, which included p27(kip1) expression, Ki-67 (MIB1) expression and all the pre-operative and post-operative parameters, showed that pelvic lymph node involvement and Ki-67 (MIB1) IE were independent prognostic markers of biochemical failure after radical prostatectomy.
在接受了根治性前列腺切除术的临床局限性前列腺癌患者中，评估了参与细胞周期调控和细胞增殖的p27（kip1）和Ki-67（MIB-1）的免疫组化表达（IE）及其预测生化失败的能力。此外，p27（kip1）和Ki-67（MIB1）表达与一些术前和术后参数相关，如Gleason评分、包膜外扩展、精囊受累、盆腔淋巴结转移、手术切缘阳性、高级别前列腺上皮内瘤变共存、肿瘤大小、前列腺体积和PSA水平。我们的分析涉及130例连续的根治性前列腺切除标本。记录到低p27（kiP1）IE与精囊受累、肿瘤体积增加和术前高PSA值之间存在统计学显著相关性。低p27（kiP1）IE与根治性前列腺切除术后生化失败的可能性增加显著相关。此外，Ki-67（MIB1）IE增加与盆腔淋巴结转移疾病显著相关，并且是生化失败的重要预测指标。包括p27（kip1）表达、Ki-67（MIB1）表达以及所有术前和术后参数的Cox回归分析表明，盆腔淋巴结受累和Ki-67（MIB1）IE是根治性前列腺切除术后生化失败的独立预后标志物。
