Jorabchi Kaveh, Kahen Kaveh, Lecchi Paolo, Montaser Akbar
Department of Chemistry, George Washington University, Washington, D.C. 20052, USA.
Anal Chem. 2005 Aug 15;77(16):5402-6. doi: 10.1021/ac0503299.
A high efficiency nebulizer (HEN) coupled to a heated spray chamber and a membrane desolvator is used for liquid sample introduction in chemical reaction interface mass spectrometry (CRIMS). Compared to the conventional thermospray nebulizer operated at solvent flow rate of 1 mL/min, the HEN provides small droplets at lower flow rates (10-100 microL/min), improving the desolvation and analyte transport efficiency. As a result, the sensitivity for carbon detection by CRIMS is improved by a factor of 4. The new arrangement offers an easy-to-use and robust interface, facilitating the availability of a variety of liquid chromatographic techniques to the CRIMS. Separation and detection of labeled peptides in a mixture of unlabeled biopolymers is illustrated at a solvent flow rate of 45 microL/min as an example of new possibilities offered by the improved liquid introduction interface.
一种与加热喷雾室和膜去溶剂化器相连的高效雾化器（HEN）用于化学反应界面质谱（CRIMS）中的液体样品引入。与以1 mL/min的溶剂流速运行的传统热喷雾雾化器相比，HEN在较低流速（10 - 100微升/分钟）下产生小液滴，提高了去溶剂化和分析物传输效率。结果，CRIMS检测碳的灵敏度提高了4倍。这种新配置提供了一个易于使用且坚固的接口，便于将各种液相色谱技术应用于CRIMS。以45微升/分钟的溶剂流速为例，展示了在未标记生物聚合物混合物中分离和检测标记肽，这体现了改进后的液体引入接口带来的新可能性。
