Matuschek Michaela C, Hendriks Wouter H, McGhie Tony K, Reynolds Gordon W
Institute of Food, Nutrition, and Human Health, Massey University, 11 222 Palmerston North, New Zealand.
J Nutr Biochem. 2006 Jan;17(1):31-6. doi: 10.1016/j.jnutbio.2005.04.005. Epub 2005 Aug 10.
Intestinal absorption of anthocyanins (ACNs) was studied in vitro by comparing ACN disappearance from the mucosal solution of Ussing chambers not containing any tissue (controls) and that of Ussing chambers containing segments of mouse duodenum, jejunum, ileum or colon. The tissues were mounted in the chambers and bathed with Ringer's solution (RS) adjusted to a pH representative of the respective segments in vivo. The chambers were kept at 37 degrees C and RS was perfused continuously with carbogen (95% O(2)/5% CO(2)). After the addition of an ACN extract to the mucosal solution, samples from both the mucosal side and the serosal side were withdrawn at 10, 40, 80 and 120 min and analyzed for ACN concentration using reversed-phase HPLC with photodiode array detection. The highest absorption of ACNs occurred in chambers mounted with jejunal tissue (max absorption rate, 55.3+/-7.6%). Minor absorption occurred with duodenal tissue (10.4+/-7.6%), with no absorption recorded when tissues from the ileum or colon were used. This study demonstrates for the first time that ACN absorption in mice occurs predominantly in the jejunum.
通过比较花青素（ACNs）在不含任何组织的尤斯灌流小室（对照组）黏膜溶液中的消失情况以及在含有小鼠十二指肠、空肠、回肠或结肠段的尤斯灌流小室中的消失情况，对ACNs的肠道吸收进行了体外研究。将组织安装在小室中，并用调整至代表体内各段相应pH值的林格氏溶液（RS）进行灌流。小室保持在37摄氏度，RS用混合气（95% O₂/5% CO₂）持续灌流。在向黏膜溶液中添加ACN提取物后，在10、40、80和120分钟时从黏膜侧和浆膜侧取出样品，使用带光电二极管阵列检测的反相高效液相色谱法分析ACN浓度。ACNs的最高吸收发生在安装有空肠组织的小室中（最大吸收率，55.3±7.6%）。十二指肠组织有少量吸收（10.4±7.6%），使用回肠或结肠组织时未记录到吸收。这项研究首次证明小鼠体内的ACN吸收主要发生在空肠。
