Shaw Brian D, Upadhyay Srijana
Program for the Biology of Filamentous Fungi, Department of Plant Pathology and Microbiology, Texas A and M University, 2132 TAMU, College Station, TX 77843, USA.
Fungal Genet Biol. 2005 Oct;42(10):862-72. doi: 10.1016/j.fgb.2005.06.002.
The Aspergillus nidulans swoK1 mutant is defective in polarity maintenance when grown at restrictive temperature (38 degrees C). Upon germination, the mutant extends a primary germ tube that swells to an enlarged, non-uniform cell with pronounced wall thickenings. The mutant is fully restored to wild-type growth when transformed with a plasmid containing the AN5802.2 ORF as designated in The Broad Institute A. nidulans sequence database. Genetic mapping places swoK in the same region of chromosome I, as that occupied by An5802.2 on the physical map. swoK is predicted to encode a protein that contains an N-terminal RRM (RNA Recognition Motif) and a highly repetitive C-terminus with numerous RD/DR and RS/SR dipeptides. We hypothesize that SwoK participates in one of the known functions of SR proteins (those that contain SR/RS repeats): mRNA maturation through the spliceosome and or transport of mRNAs out of the nucleus to sites of protein translation.
构巢曲霉swoK1突变体在限制温度（38摄氏度）下生长时，极性维持存在缺陷。萌发后，该突变体伸出一条初级芽管，其膨胀成为一个增大的、不均匀的细胞，伴有明显的细胞壁增厚。当用含有布罗德研究所构巢曲霉序列数据库中指定的AN5802.2开放阅读框的质粒转化时，该突变体完全恢复为野生型生长。遗传定位将swoK定位于染色体I的同一区域，该区域在物理图谱上由An5802.2占据。预测swoK编码一种蛋白质，该蛋白质包含一个N端RRM（RNA识别基序）和一个高度重复的C端，带有许多RD/DR和RS/SR二肽。我们假设SwoK参与SR蛋白（那些包含SR/RS重复序列的蛋白）的已知功能之一：通过剪接体进行mRNA成熟和/或将mRNA从细胞核转运到蛋白质翻译位点。
