School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Integrative Biology, Kyungpook National University; Daegu 41566, Republic of Korea.
J Microbiol Biotechnol. 2021 May 28;31(5):676-685. doi: 10.4014/jmb.2101.01011.
RNA-binding proteins are involved in RNA metabolism and posttranscriptional regulation of various fundamental biological processes. The PUF family of RNA-binding proteins is highly conserved in eukaryotes, and its members regulate gene expression, mitochondrial biogenesis, and RNA processing. However, their biological functions in species remain mostly unknown in filamentous fungi. Here we have characterized the puf genes in the model organism . We generated deletion mutant strains for the five putative puf genes present in the genome and investigated their developmental phenotypes. Deletion of or affected fungal growth and asexual development. mutants exhibited decreased production of asexual spores and reduced mRNA expression of genes regulating asexual development. The deletion reduced colony growth, increased formation of asexual spores, and delayed production of sexual fruiting bodies. In addition, the absence of reduced both sterigmatocystin production and the mRNA levels of genes in the sterigmatocystin cluster. Finally, deletion mutants showed reduced trehalose production and lower resistance to thermal stress. Overall, these results demonstrate that PufA and PufE play roles in the development and sterigmatocystin metabolism in .
RNA 结合蛋白参与 RNA 代谢和各种基本生物过程的转录后调控。RNA 结合蛋白的 PUF 家族在真核生物中高度保守，其成员调节基因表达、线粒体生物发生和 RNA 加工。然而，它们在丝状真菌中的生物学功能在物种中仍然知之甚少。在这里，我们对模式生物 中的 puf 基因进行了表征。我们生成了 5 个假定存在于 基因组中的 puf 基因的缺失突变株，并研究了它们的发育表型。缺失 或 影响真菌生长和无性发育。 突变体产生的无性孢子减少，调节无性发育的基因的 mRNA 表达降低。 缺失减少了菌落生长，增加了无性孢子的形成，并延迟了有性生殖体的产生。此外， 的缺失减少了麦角甾酮的产生和麦角甾酮簇中基因的 mRNA 水平。最后， 缺失突变体表现出较低的海藻糖产量和对热应激的抵抗力降低。总的来说，这些结果表明 PufA 和 PufE 在 的发育和麦角甾酮代谢中发挥作用。
