Ihalainen Janne A, Klimmek Frank, Ganeteg Ulrika, van Stokkum Ivo H M, van Grondelle Rienk, Jansson Stefan, Dekker Jan P
Vrije Universiteit Amsterdam, Department of Physics and Astronomy, Biophysics, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.
FEBS Lett. 2005 Aug 29;579(21):4787-91. doi: 10.1016/j.febslet.2005.06.091.
We report a time-resolved fluorescence spectroscopy characterization of photosystem I (PSI) particles prepared from Arabidopsis lines with knock-out mutations against the peripheral antenna proteins of Lhca1 or Lhca4. The first mutant retains Lhca2 and Lhca3 while the second retains one other light-harvesting protein of photosystem I (Lhca) protein, probably Lhca5. The results indicate that Lhca2/3 and Lhca1/4 each provides about equally effective energy transfer routes to the PSI core complex, and that Lhca5 provides a less effective energy transfer route. We suggest that the specific location of each Lhca protein within the PSI-LHCI supercomplex is more important than the presence of so-called red chlorophylls in the Lhca proteins.
我们报道了对从拟南芥品系制备的光系统I（PSI）颗粒的时间分辨荧光光谱表征，这些拟南芥品系针对Lhca1或Lhca4外周天线蛋白具有敲除突变。第一个突变体保留Lhca2和Lhca3，而第二个突变体保留光系统I（Lhca）的另一种捕光蛋白，可能是Lhca5。结果表明，Lhca2/3和Lhca1/4各自为PSI核心复合体提供了大致同等有效的能量传递途径，而Lhca5提供的能量传递途径效率较低。我们认为，每个Lhca蛋白在PSI-LHCI超复合体中的特定位置比Lhca蛋白中所谓的红色叶绿素的存在更为重要。
