Tripathi Vijay Shyam, Kandimalla Vivek Babu, Ju Huangxian
Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China), Department of Chemistry, Nanjing University.
Biosens Bioelectron. 2006 Feb 15;21(8):1529-35. doi: 10.1016/j.bios.2005.07.006. Epub 2005 Aug 11.
A novel amperometric biosensor for hydrogen peroxide (H(2)O(2)) was developed by entrapping horseradish peroxidase (HRP) in a new ormosil composite doped with ferrocene monocarboxylic acid-bovine serum albumin conjugate and multiwall carbon nanotubes (MWNTs). The ormosil was prepared using 3-(aminopropyl)triethoxysilane and 2-(3,4 epoxycyclohexyl)-ethyltrimethoxy silane as monomers. The encapsulated conjugate showed excellent electrochemistry and acted as an electron transfer mediator. The presence of MWNTs improved the conductivity of the composite film. This matrix showed a biocompatible microenvironment for retaining the native activity of the entrapped HRP and a very low mass transport barrier to the substrate, which provided a fast amperometric response to H(2)O(2). The proposed H(2)O(2) biosensor exhibited a linear range of 0.02-4.0 mM with a detection limit of 5.0 microM (S/N = 3) and a K(M)(app) value of 2.0 mM. It could be used for flow injection analysis of hydrogen peroxide with a liner range from 0.02 to 4.5 mM, sensitivity of 0.042 microA/mM and analytical time of 20 s per sample. This biosensor possessed good analytical performance and storage stability.
通过将辣根过氧化物酶（HRP）包埋在掺杂二茂铁单羧酸 - 牛血清白蛋白共轭物和多壁碳纳米管（MWNTs）的新型有机硅复合材料中，开发了一种用于过氧化氢（H₂O₂）的新型安培生物传感器。该有机硅使用3 - （氨丙基）三乙氧基硅烷和2 - （3,4 - 环氧环己基） - 乙基三甲氧基硅烷作为单体来制备。包封的共轭物表现出优异的电化学性能，并充当电子转移介质。MWNTs的存在提高了复合膜的导电性。这种基质为保留包埋的HRP的天然活性提供了生物相容性微环境，并且对底物具有非常低的传质屏障，这为H₂O₂提供了快速的安培响应。所提出的H₂O₂生物传感器的线性范围为0.02 - 4.0 mM，检测限为5.0 μM（S/N = 3），表观米氏常数（Kₘₐₚ）值为2.0 mM。它可用于过氧化氢的流动注射分析，线性范围为0.02至4.5 mM，灵敏度为0.042 μA/mM，每个样品的分析时间为20秒。这种生物传感器具有良好的分析性能和储存稳定性。
